ALDOSE REDUCTASE AND DIABETIC COMPLICATIONS

醛糖还原酶是多基因醛酮还原酶家族的一员， 与糖尿病并发症的病因有关， 影响许多组织，但特别是眼睛、神经和肾脏。 大多数先前 醛糖还原酶的研究，包括开发醛糖还原酶 抑制剂作为潜在的治疗药物，已经利用动物来源的 醛糖还原酶，其性质与人的醛糖明显不同 还原酶。 最近，开发了允许分离人类 醛糖还原酶而不改变其天然性质。 的 本研究的目的是分析动力学和药物结合 人醛糖还原酶的性质，以建立一个坚定的 酶的基础上，开发未来的治疗药物。 具体目标如下： 1.为了确定是否多种形式的人类醛糖还原酶 代表不同的基因产物。 2.分析多种形式的人醛糖的动力学性质 还原酶，重点是葡萄糖作为底物。 3.分析人醛糖还原酶的药物结合特性。

Aldose reductase, a member of the multi-gene aldo-keto reductase family, has been implicated in the etiology of diabetic complications which can affect many tissues, but especially eye, nerve and kidney. Most previous studies of aldose reductase, including development of aldose reductase inhibitors as potential therapeutic drugs, have utilized animal sources of aldose reductase, whose properties differ markedly from human aldose reductase. Recently, methods were developed that allow isolation of human aldose reductase without alteration of its native properties. The objective of the study is to analyze the kinetic and drug binding properties of human aldose reductase in order to establish a firm enzymological foundation upon which to develop future therapeutic drugs. The Specific Aims are as follows: 1. To determine whether the multiple form of human aldose reductase represent different gene products. 2. To analyze the kinetic properties of the multiple forms of human aldose reductase with emphasis on glucose as substrate. 3. To analyze the drug binding properties of human aldose reductase.