GENETICS OF HEAT SHOCK IN MYCOPLASMA GENITALIUM

支原体是一种致病的无壁细菌。 在人类中，低等动物、植物和节肢动物。虽然不是致命的，但它们已经 与许多与免疫功能受损有关的疾病有关 宿主--包括艾滋病。遗传学和生理学研究 这种生物体由于无法获得稳定的 基因突变，以及缺乏遗传转移系统。压力 反应是自然界中最保守的反应，一直以来 在所有被测试的生物体中都发现了这种物质--从细菌到植物再到人类。vt.在.的基础上 在应激刺激下，细胞迅速合成一组从头开始的蛋白质。 热休克蛋白家族是最丰富和最保守的 自然界中的一组蛋白质。应激反应显著 在为传染病提供第一道防线方面的影响， 自身免疫学和免疫病理学。建议的总体目标是 这项研究旨在为解开M。 生殖器。由于这种有机体的编码能力有限，它 为确定最少的基因数量提供了一个极好的模型 自我存在和/或致病性所必需的。具体目标有：1) 之前完成了四个热休克基因的DNA序列 识别，2)将这些基因映射到最近发布的物理图谱 3)扩充已知的生殖支原体热休克基因库。

Mycoplasmas (class Mollicutes) are wall-less bacteria that are pathogenic in man, lower animals, plants and arthropods. Though not fatal, they have been associated with a number of diseases involved with immunocompromised hosts -- including AIDS. Genetic and physiological research studies for this organism have been hampered due to the inability to obtain stable genetic mutants, and the lack of a genetic transfer system. Stress response is the most highly conserved response in nature and has been found in all organisms tested -- from bacteria to plants to humans. Upon stressful stimuli, cells rapidly synthesize a de novo set of proteins. The heat shock family of proteins is the most abundant and most conserved set of proteins in nature. The stress response has significant implications in providing a first line of defense for infectious diseases, autoimmunity, and immunopathology. The overall objective of the proposed study is to provide initial studies for unraveling this response in M. genitalium. Because of this organism's limited coding capacity, it provides an excellent model for determining the least number of genes required for self existence and/or pathogenicity. Specific aims are: 1) to complete the DNA sequence of four heat shock genes previously identified, 2) to map these genes to the recently published physical map and 3) to expand the repertoire of known M. genitalium heat shock genes.