RETROVIRUSES--RECOMBINATION/RETROFECTION/PATHOGENESIS

这项研究的目标是获得培训和专业知识 逆转录病毒的分子生物学及其最终的应用 猫科动物免疫缺陷发病机制研究的体会 病毒(FIV)。该计划的第一阶段致力于学习各种 分子病毒学在禽类研究中的技术和方法 逆转录病毒。在此初始阶段，将在以下方面获得经验 组织培养以及DNA、RNA和蛋白质操作。这 阶段将重点放在逆转录病毒粒子的能力上 通过感染将所需的表型传递给受体细胞，以及 异源重组是逆转录病毒和 非逆转录病毒序列。这项研究的一部分将用于实现 微生物学博士学位论文要求。该项目的第二阶段 该计划将包括应用分子方法和 在第一阶段学到的技术用于研究FIV的向向性和 发病机制。FIV是最近发现的一种慢病毒，它能引起 猫的免疫缺陷疾病。它也是一种很好的动物模型 艾滋病。FIV的分子克隆将直接从猫组织中获得 并在第二阶段进行体外鉴定。猫的未来实验 将由这些研究演变而来，并将成为未来拨款的重点 申请。第一阶段和第二阶段的研究将在 对分子领域中地位显赫的个人的监督 逆转录病毒学。

The goal of this study is to obtain training and expertise in the molecular biology of retroviruses with the eventual application of such experience to the study of the pathogenesis of feline immunodeficiency virus (FIV). Phase I of this plan is devoted to learning the various techniques and approaches used in molecular virology while studying avian retroviruses. During this initial phase, experience will be obtained in tissue culture as well as DNA, RNA, and protein manipulations. This phase will focus on retrofection, or the ability of retroviral particles to transmit desired phenotypes to recipient cells by infection, and on heterologous recombination which is recombination between retroviral and non-retroviral sequences. Part of this research will be used to fulfill thesis requirements for a Ph.D. degree in Microbiology. Phase II of this program will involve application Of the molecular approaches and techniques learned in Phase I to the study of FIV tropism and pathogenesis. FIV is a recently discovered lentivirus that causes immunodeficiency disease in cats. It is also a good animal model for AIDS. Molecular clones of FIV will be obtained directly from cat tissues and characterized in vitro during Phase II. Future experiments in cats will evolve from these studies and will be the focus of future grant applications. The studies in Phases I and II will be carried out under the supervision of well-established individuals in the field of molecular retrovirology.