CONTROL OF LHRH SECRETION IN THE MALE

申请者已经证明，睾酮(T)作用于大脑，以 降低促性腺激素释放激素(GnRH)脉冲频率。这个 拟议研究的总体目标是检验一般假设 T抑制男性GnRH的脉冲频率，部分是通过作用 在医学视前区(MPOA)增加伽马分泌 氨基丁酸(GABA)，并减少谷氨酸和 天冬氨酸、兴奋性氨基酸(EAA)。 具体目标是： 1.检验T对GNRN的抑制作用假设 通过作用于mPOA的脉冲频率。这将通过以下方式解决： 双侧睾丸激素植入mPOA，弓状核- 腹内侧区(ARC-VMR)，以及同时存在的两个区域，然后 监测黄体生成素脉象在切除或切除后的变化 (奥奇)公羊。 2.检验GABA抑制GnRH脉冲频率的假设 公羊。这将通过灌流GABA激动剂和拮抗剂来实现。 进入mPOA，然后监测Orch的黄体生成素脉冲模式的变化 还有奥奇，T处理过的公羊。 3.验证EAAS增加GnRH脉冲频率的假说。这 将EAA激动剂和拮抗剂灌流到mPOA中 奥奇和T处理奥奇公羊，然后监测黄体生成素脉搏的变化 频率 4.验证T升高GABA和减少EAA分泌的假说 在mpoa里。这将通过监测GABA和EAA的释放来完成 采用推拉式插管同时监测循环的POA 用T或稀释剂处理的奥奇公羊的促黄体生成素。 5.检验零假设，即两者几乎没有重合 MPOA中GABA和EAA的分泌以及GnRH释放的振荡。 血浆中GABA、EAA、GnRH、GnRH、GnRH和GnRH的动态变化 而促黄体生成素的分泌将在Orch和Orch+T处理的公羊中完成。 这些研究对于理解GnRH的控制是很重要的 脉冲发生系统与T对促性腺激素释放激素和黄体生成素的调节作用 分泌物。

The applicant has shown that testosterone (T) acts on the brain to decrease gonadotropin releasing-hormone (GnRH) pulse frequency. The overall aim of the proposed studies is to test the general hypothesis that T suppresses GnRH pulse frequency in the male, in part, by acting at the medical preoptic area (mPOA) to increase secretion of gamma aminobutyric acid (GABA), and decrease secretion of glutamate and aspartate, excitatory amino acids (EAA). The specific aims are: 1. To test the hypothesis that T exerts its inhibitory effect on GNRN pulse frequency by acting at the mPOA. This will be addressed by placing bilateral microimplants of the testosterone into the mPOA, the arcuate- ventromedial region (ARC-VMR), and both regions simultaneously, and then monitoring subsequent changes of LH pulse patterns in or chidectomized (ORCH) rams. 2. To test the hypothesis that GABA suppresses GnRH pulse frequency in the ram. This will be done by perfusing GABA agonists and antagonists into the mPOA and then monitoring changes of LH pulse patterns in ORCH and ORCH, T-treated rams. 3. To test the hypothesis that EAAs increase GnRH pulse frequency. This will be done by perfusing EAA agonists and antagonists into the mPOA of oRCH and T-treated ORCH rams and then monitoring changes in LH pulse frequency. 4. To test the hypothesis that T elevates GABA and reduces EAA secretion in the mPOA. This will be done by monitoring GABA and EAA release from the POA using push-pull cannule and simultaneously monitoring circulating LH in ORCH rams treated with T or diluent. 5. To test the null hypothesis that there is little coincidence between oscillations of GABA, and EAA secretion in the mPOA and of GnRH release. A longitudinal study of simultaneous temporal changes of GABA, EAA, GnRH, and LH secretion will be done in ORCH and ORCH + T-treated rams. These studies are important for understanding both control of the GnRH pulse-generating system and the action of T in regulating GnRH and LH secretion.