ORAL IMPLICATIONS OF HUMAN HERPES VIRUS 8 (HHV8)

描述：人类疱疹病毒8型（HHV-8）已在卡波西斯被发现 肉瘤（KS）以及各种其他组织，包括来自 口腔。 该建议试图确定1）在细胞中的细胞类型， 口腔粘膜支持HHV-8感染，2）细胞的依赖性， 3）口服HHV-8与病毒复制的相关性 口腔KS感染和口腔溃疡; 4）HIV-1的作用 共同感染增强HHV-8活性和5）评估唾液作为 HHV-8传输的车辆。 原位杂交和原位PCR 技术将被用于识别携带HHV-8和HIV-1的细胞 基因组和分化上皮细胞的培养物中。 有限 将对HIV-1阳性患者进行队列和研究，以确定 与口腔KS发展相关的参数。

DESCRIPTION: Human herpes virus 8, (HHV-8) has been identified in Kaposis sarcoma (KS) as well as in a variety of other tissues including those from the oral cavity. This proposal seeks to determine 1) the cell types in the oral mucosa that support HHV-8 infection, 2) the dependency of cellular differentiation on viral replication, 3) the association of oral HHV-8 infection with oral KS and oral ulceration, 4) the role of HIV-1 co-infection in enhancement of HHV-8 activity and 5) evaluate saliva as a vehicle of HHV-8 transmission. In-situ hybridization and in-situ PCR techniques will be used to identify the cells harboring HHV-8 and HIV-1 genomes and in the cultures of differentiating epithelial cells. A limited cohort and study will be done on HIV-1 positive patients to determine parameters associated with the development of oral KS.