OPERATIONAL MECHANISMS OF AIRWAY RECEPTORS

拟议中的调查将直接进行， 继续我们先前关于喉和上呼吸道感受器的工作， 和相关的反射反应。 根据以前关于 水溶液对喉感受器活性的影响，我们将研究 通过以下方式改变喉反射反应的强度的可能性 粘膜表面液体的物理化学组成的变化 (Aim#1）。 胃食管反射是一种相对常见的疾病， 越来越多地被认为是一个致病因素，在发展中国家， 许多喉部病变。 我们将研究盐酸胃蛋白酶的作用 解决方案适用于喉粘膜的神经功能，这 器官：受体转导和相关的反射反应。 组织学 将对喉粘膜和粘膜下层进行控制， 表征损伤（炎症、坏死等）由于暴露于 HCl-胃蛋白酶溶液（目标#2）。 喉部预计将是最 整个呼吸道的反应性，tussigenic部位和一些证据 赞成这种期望。 然而，相反的证据也存在 available. 使用一种制剂，其中上气道和下气道 在功能上隔离，我们将比较咳嗽的诱发性和 其对可比机械和化学刺激的努力强度 喉部和气管支气管树。 此外，我们将评估 喉内肌及其颞肌的放电模式 与喉声门下压变化的关系， 气管支气管咳嗽 我们亦会研究是否有可能更改 喉肌放电模式，在气管支气管诱发咳嗽， 在喉部传入神经切断后 的发展方面 喉和气管支气管对伤害性刺激的反应性 研究了

The investigations being proposed will be carried out in direct continuation of our previous work on laryngeal and upper airway receptors, and related reflex responses. On the basis of previous observations on the effect of water solutions on laryngeal receptors activity, we will examine the possibility of modifying the strength of laryngeal reflex responses by changes in the physico-chemical composition of the mucosal surface liquid (Aim #1). A relatively common disorder, gastroesophageal reflex, has increasingly been recognized as an etiologic factor int he development of many laryngeal lesions. We will examine the effects of a HCI-pepsin solution applied to the laryngeal mucosa on the neural function of this organ: receptor transduction and related reflex responses. Histological control of the laryngeal mucosa and submucosa will be carried out to characterize the damage (inflammation, necrosis...) due to the exposure to HCI-pepsin solutions (Aim #2). The larynx is expected to be the most reactive, tussigenic-site of the entire respiratory tract and some evidence favors this expectation. However, evidence to the contrary is also available. Using a preparation in which upper and lower airway are functionally isolated, we will compare the elicitability of cough and the intensity of its efforts to comparable mechanical and chemical irritation of the larynx and the tracheobronchial tree. Moreover, we will evaluate the pattern of discharge of intrinsic laryngeal muscles and their temporal relationship to changes in subglottic pressure in laryngeal and tracheobronchial cough. We will also study any possible alteration of laryngeal muscle pattern of discharge, in tracheobronchial-elicted cough, following deafferentation of the larynx. Developmental aspects of laryngeal and tracheobronchial reactivity to noxious stimuli will be studied.