95F MYOSIN FUNCTION IN DROSOPHILA NEURONS

该建议的重点是确定一种抗肿瘤药物的体内功能。 非常规肌球蛋白，95 F肌球蛋白，在果蝇神经系统中。95F 肌球蛋白的表达在整个生命周期中受到发育调控 这样一些组织，包括神经系统，表达 相对较高的蛋白质水平。 初步证据 证明95 F肌球蛋白具有很强的定位性， 微粒性质的神经细胞过程的中枢神经系统。 因为95 F肌球蛋白已经被证明是一个马达， 早期胚胎中的细胞质颗粒， 神经元可能表明95 F肌球蛋白与颗粒相关， 负责他们的运输。因此，我建议 研究95 F肌球蛋白在发育中的神经元中的体内功能， 文化我计划分析95 F肌球蛋白介导的运动和95 F肌球蛋白 与GFP-95 F肌球蛋白融合的体内分布。我也会检查 95 F肌球蛋白突变的影响，以确定95 F肌球蛋白是否是 对于神经元发育和/或神经元内的运输是重要的。 最后，我计划确定含有95 F肌球蛋白的 在成熟神经元中的颗粒，并进行95 F肌球蛋白的详细分析 胚胎神经系统中的蛋白质分布。 拟议 实验将提供有关体内功能的新信息， 95 F肌球蛋白，以及肌动蛋白为基础的运输机制， 神经元

The focus of this proposal is to determine the in vivo function of an unconventional myosin, 95F myosin, in the Drosophila nervous system. 95F myosin expression is developmentally regulated throughout the life cycle of the fly such that some tissues, including the nervous system, express comparatively higher levels of protein. Preliminary evidence demonstrates that 95F myosin exhibits strong localization of a particulate nature to nerve cell processes of the central nervous system. Because 95F myosin has been shown to be a motor that transports cytoplasmic particles in the early embryo, the particulate staining in neurons may indicate that 95F myosin is associated with particles and may be responsible for their transport. Therefore, I am proposing to investigate the in vivo function of 95F myosin in developing neurons in culture. I plan to analyze 95F myosin-mediated motility and 95F myosin distribution in vivo with a GFP-95F myosin fusion. I will also examine the effects of mutations in 95F myosin to determine whether 95F myosin is important for neuronal development and/or transport within neurons. Finally, l plan to determine the nature of 95F myosin-containing particles in mature neurons and perform detailed analysis of 95F myosin protein distribution in the embryonic nervous system. The proposed experiments will provide new information about the in vivo functions of 95F myosin, as well as the actin-based transportation mechanisms in neurons.