ASTROGLIOSIS AND EPILEPTOGENESIS

虽然神经胶质瘢痕形成长期以来与局灶性癫痫有关， 迄今为止的研究未能建立病理生理联系。 越来越多的证据表明，中枢神经系统 星形胶质细胞在调节细胞外离子 稳态和细胞外间隙（ECS）大小对神经元 活动 初步结果表明，改变不同的神经胶质细胞， 调节细胞外环境的机制要么会导致 神经元同步性增加或减少。 正常 海马切片，阻断钾摄取进入神经胶质细胞， 癫痫样活动 相反，防止ECS收缩拮抗 癫痫样活动在体外和急性动物模型， 癫痫 我们建议研究星形胶质细胞对细胞外 海人酸大鼠慢性颞叶癫痫模型中的环境 以及颞叶癫痫切除术的人类标本。 我们将 检验钾调节改变的假设和/或 红藻氨酸诱导胶质化海马星形胶质细胞合胞体的ECS大小 处理过的大鼠和来自颞叶癫痫的人类手术标本 切除术

Although glial scarring has long been associated with focal epilepsy, studies to date have failed to establish a pathophysiologic linkage. There is increasing evidence however that central nervous system astrocytes play an essential role in regulating extracellular ion homeostasis and extracellular space (ECS) size in response to neuronal activity. Preliminary results have shown that altering different glial mechanisms that regulate the extracellular environment can either result in an increase of decrease of neuronal synchronization. In normal hippocampal slices, blocking potassium uptake into glia results in epileptiform activity. In contrast, preventing ECS shrinkage antagonizes epileptiform activity in both in vitro and acute animal models of epilepsy. We propose to study astrocytic regulation of the extracellular environment in the kainate rat model of chronic temporal lobe epilepsy and in human specimens from temporal lobed epilepsy resections. We will test the hypothesis that there is altered regulation of potassium and/or ECS size by the astrocyte syncytium in gliotic hippocampus from kainate treated rats and human surgical specimens from temporal lobe epilepsy resections.