CORNEAL INFLAMMATION AND INTERLEUKIN 10

描述（改编自申请人的摘要）：本文的重点 该项目旨在研究常驻角膜细胞与 免疫调节细胞因子IL-10。 研究将与 培养的人和鼠角膜上皮细胞和成纤维细胞。 RT- PCR分析将用于精确确定哪种常驻角膜 细胞表达IL-10受体mRNA。 流式细胞术和免疫组织学 将被用于搜索不同角膜上的蛋白质产物 细胞类型。 白细胞介素-10与其受体结合对角膜上皮细胞的影响 细胞将从确定是否 配体结合导致抑制的细胞因子/趋化因子mRNA和/或 蛋白质合成 我们实验室先前的RT-PCR和ELISA研究 显示IL-10抑制IL-6 mRNA和蛋白质合成。 的 申请人假设IL-10抑制细胞因子合成， 基因转录水平或干扰mRNA稳定性。 初步数据表明，人类和小鼠角膜具有mRNA， IL-10。 申请人将测试常驻角膜细胞是否使 IL-10蛋白。 阳性结果表明IL-10可能在 作为常驻角膜细胞的自动调节因子。 基本 这些研究产生的信息可能有助于设计新的 控制眼部炎症的方法。

DESCRIPTION (Adapted from applicant's abstract): The focus of this project is to investigate resident corneal cell interaction with the immunoregulatory cytokine IL-10. Studies will be carried out with cultured human and murine corneal epithelial cells and fibroblasts. RT- PCR analysis will be used to determine precisely which resident corneal cells express IL-10 receptor mRNA. Flow cytometry and immunohistology will be used to search for the protein product on the different corneal cell types. The consequences of IL-10 binding to its receptor on corneal cells will be analyzed from the perspective of determining whether ligand binding leads to suppressed cytokine/chemokine mRNA and/or protein synthesis. Prior RT-PCR and ELISA studies in our laboratory have shown that IL-10 suppresses IL-6 mRNA and protein synthesis. The applicant hypothesizes that IL-10 inhibits cytokine synthesis at the level of gene transcription or interferes with mRNA stability. Preliminary data indicate that human and mouse corneas possess mRNA for IL-10. The applicant will test whether resident corneal cells make IL-10 protein. Positive results would suggest that IL-10 may function as an autoregulatory factor for resident corneal cells. The basic information generated by these studies may prove useful for devising new approaches to control ocular inflammation.