INSTRUMENTATION FOR ANALYSIS OF HIGH MASS BIOMOLECULES

描述：这项工作的目标是推动- 生物分子的电喷雾/离子陷阱质谱学研究 也提高了仪器的分析优值系数 通过发展新的实验来获得高质量的信息 与生物相关的离子。 四极离子陷阱是一种非常强大的工具 生物衍生离子的操纵和质量/电荷分析。 此外，它的成本远远低于其他形式的 能够进行串联质谱分析的仪器 实验。后一种特性使离子陷阱具有潜在的 生物医学领域的广泛研究人员都可以使用。这 工作致力于实现对高质量的超低水平分析/检测 生物分子(低分子水平)，无预浓缩，常见 在线毛细管电泳和流动等分析场景 注射分析。我们将特别强调如何使用 非破坏性离子检测和重新测量的背景下 多阶段质谱学实验。存储离子的能力 将用于开发离子/分子反应的新应用，以及 探索与多电荷反应有关的化学 具有相反电荷离子的生物聚合物。离子/分子反应 应用包括一种增加离子电流的技术 通过质子将多电荷生物聚合物提高几个数量级 转移反应和电喷雾的处理方法 串联质谱学和质子转移的混合物 反应。离子/离子反应研究将集中在两个质子转移 和电子转移，特别注意化学 通过电子转移反应形成的奇电子物种。在……里面 所有情况下，重点都将放在能够提供新的 信息或可以增强离子陷阱性能的信息 当前的方法。

DESCRIPTION: The objective of this work is to advance the state-of-the- art in electrospray/ion trap mass spectrometry of biomolecules by improving the analytical figures of merit of the instrumentation as well as by developing new experiments to gain information on high mass biologically relevant ions. The quadrupole ion trap is a remarkably powerful tool for the manipulation and mass/charge analysis of biologically-derived ions. Furthermore, it is far less expensive than other forms of instrumentation capable of performing tandem mass spectrometric experiments. The latter characteristic makes the ion trap potentially accessible to a wide range of researchers in the biomedical field. This work seeks to achieve ultra-low level analysis/detection of high mass biomolecules (low attomole levels), without preconcentration, in common analytical scenarios such as on-line capillary electrophoresis and flow injection analysis. Particular emphasis will be placed on the use of non-destructive ion detection and re-measurement within the context of multi-stage mass spectrometry experiments. The ability to store ions will be used to develop new applications for ion/molecule reactions and to explore the chemistry associated with reactions of multiply-charged biopolymers with oppositely charged ions. The ion/molecule reaction applications include a technique to increase ion currents derived from multiply-charged biopolymers by several orders of magnitude via proton transfer reactions and an approach to dealing with electrospray of mixtures involving tandem mass spectrometry and proton transfer reactions. Ion/ion reaction studies will focus on both proton transfer and electron transfer with particular attention paid to the chemistry of the odd-electron species formed via electron transfer reactions. In all cases, emphasis will be placed on experiments that can provide new information or that can enhance the performance of the ion trap over current methodology.