NEURAL BASIS OF TASTE ELICITED INGESTION AND REJECTION

描述：(改编自申请人的摘要)。目标是 这项拟议的研究的主要目的是确定 通过味觉刺激引导进食的啮齿动物后脑 行为。尽管对摄食行为的监管过于苛刻 复杂，累及中枢神经系统的广泛区域， 味觉的基本调节功能决定了它的可口性 在尾部头脑风暴中，令人不快的刺激是完整的。这个 正常情况下，对可口和难吃的区分是显而易见的 去大脑动物对味觉刺激的摄取和排斥反应 准备工作。神经解剖学研究表明，多突触通路 从孤束嘴核的味觉区发出 影响运动神经核产生消耗性成分 摄取和排斥。这些通路与脑干部位重叠。 被认为是咀嚼、舔和吞咽的一代。 在啮齿动物后脑进行的一系列慢性记录实验将 确定延髓网状结构不同区域的程度 形成涉及到味觉、引起的摄取和排斥。因为 舔、吞咽和拒绝的口腔运动功能共有 常见的口腔肌肉系统，许多相同的中间神经元在 调查预计将涉及多项职能。这个 给定神经元在多种功能中的参与程度将通过 神经活动与肌电反应的相关性 参与不同功能的肌肉的子集。时间序列分析， 尖峰计数、事件平均值和尖峰触发平均值都将 用于评估网状结构神经元的反应特性 在摄取和排斥的特定部分。一个基本原则 需要解决的问题是与脑损伤相关的神经元的身份 口述阶段的拒绝，“张口结舌”反应。这些神经元是 与神经元产生吞咽循环，吞咽循环， 呼吸神经元，还是一组独特的神经元？平行的一系列 神经解剖学研究将使用双重标记技术来识别 脑干的不同区域参与摄取和 并进一步确定它们与造山机的连通性 原子核。

DESCRIPTION: (adapted from the applicant's abstract). The objective of the proposed research is to determine the neural substrates in the rodent hindbrain through which gustatory stimuli guide ingestive behavior. Although the regulation of ingestive behavior is exceedingly complex, involving widespread regions of the central nervous system, the basic regulatory function of taste to determine palatable from unpalatable stimuli is complete in the caudal brainstorm. The discrimination of palatable from unpalatable is evident by normal ingestion and rejection responses to taste stimuli in decerebrate preparations. Neuroanatomical studies suggest that polysynaptic pathways from the gustatory region of the rostral nucleus of the solitary tract influence oromotor nuclei producing the consummatory components of ingestion and rejection. These pathways overlap with brainstem sites identified with the generation of mastication, licking and swallowing. A series of chronic recording experiments in the rodent hind brain will determine to what extent different regions of the medullary reticular formation are involved in taste elicited ingestion and rejection. Because the oromotor functions of licking, swallowing and rejection share a common oral musculature, many of the same interneurons under investigation are expected to be involved in multiple functions. The involvement of a given neuron in multiple functions will be assessed by correlating neural activity with electromyographic responses from a subset of muscles involved in diverse functions. Time series analyses, spike counts, event averaging and spike triggered averaging will all be used to assess the response properties of reticular formation neurons during specific components of ingestion and rejection. One fundamental question to be addressed is the identity of neurons associated with the oral phase of rejection, the "gape" response. Are these neurons associated with neurons generating a lick cycle, a swallow-cycle, respiratory neurons, or a unique set of neurons? A parallel series of neuroanatomical studies will use double labeling techniques to identify regions of the brainstem differentially involved in ingestion and rejection and to further determine their connectivity with the oromotor nuclei.