DIET FRUCTOSE AND GLYCATING COMPOUNDS IN HUMAN RED CELLS

食用蔗糖和高果糖形式的精制糖 玉米糖浆在美国和西方世界的价格都很高。而当 这种现象对公共卫生的影响已经被研究过了。 最近在人类中广泛发现的果糖-3-磷酸(F3P) 红细胞提示应检查人体内的果糖代谢 此外，特别强调这一新的代谢途径。是这样的 需要进行一项研究，因为初步数据表明红细胞F3P 在人体内主要由饮食中的果糖产生，而F3P是一种 一种有效的反应性酮醛3-的重要系统来源- 脱氧葡萄糖苷(3DG)。这个化合物被认为是一把钥匙这把钥匙 蛋白质非酶糖基化过程中的中间体 似乎与糖尿病并发症的病因学有关。 已公布的证据表明，这种有毒化合物是在体内产生的 在动物和人类中，通过还原为3-脱氧果糖来解毒 (3df)。 本提案旨在研究果糖-3-酮的合成细节。 人红细胞内和体外的磷酸盐与一种特殊的 强调了解解毒机制及其可能的作用 病理性损害，如高血糖。

Consumption of refined sugars in the form of sucrose and high-fructose corn syrup is very high in the U.S. and in the Western world. While the public health implications of this phenomenon have been studied extensively, the recent discovery of fructose-3-phosphate (F3P) in human erythrocytes suggests that fructose metabolism in man should be examined further, with a particular emphasis on this novel metabolic pathway. Such a study is needed because preliminary data suggest that erythrocytic F3P in man is produced primarily from dietary fructose and that F3P is an important systemic source of a potent reactive ketoaldehyde 3- deoxyglucosone (3DG). This compound is considered to be a key this a key intermediate in the process of non-enzymatic glycation of proteins which appears to be involved in the etiology of diabetic complications. Published evidence suggests that this toxic compound is produced in-vivo in animals and man and is detoxified by reduction to 3-deoxyfructose (3DF). This proposal seeks to investigate the details of synthesis of fructose-3- phosphate in human erythrocytes ex vivo and in vivo with a particular emphasis on understanding the detoxification mechanisms and their possible impairment in pathological conditions such as hyperglycemia.