MECHANISMS OF RED NUCLEUS BURST DISCHARGE

一种新型的体外制剂，它利用了海龟的不同寻常的 对缺氧的抗性已被开发出来，以探索其机制 运动项目的中心代表。这一制剂保存了 由突触连接组成的小脑-鲁滨脑回路 在小脑、红核和网状结构之间。一连串的 在表现为猴子、猫和其他动物的红核中记录的活动 海龟与运动参数如速度密切相关 似乎代表了简单的运动程序。猝发放电还可以 在体外制备中被诱导。建议的目标是 实验是为了检查细胞和电路机制， 有助于红核爆发放电的产生，以及 以此类推，运动程序的生成。胞外和胞内 结合离子替代的红核神经元的记录 并将应用活性依赖染料来研究 循环兴奋与内在膜的相对贡献 在产生爆裂放电方面的特性。机制还必须 修改移动命令，使其适应 环境。可能用于调节红核运动的机制 输出可能包括神经递质或神经调节剂，如GABA 或者5-羟色胺。对突发性放电的精细控制的研究将 用细胞外录音和离子导入检查 神经递质及其拮抗剂。这些对细胞的研究 和突触整合的电路基础在红色中潜在的活动 Nucus将提供更好地理解 小脑丘脑突触通路在适应性运动中的作用 控制和运动障碍，特别是帕金森氏症。

A novel in vitro preparation that takes advantage of the turtle's unusual resistance to anoxia has been developed to explore mechanisms of the central representation of motor programs. This preparation preserves the cerebellorubral circuit which is comprised of synaptic connections between the cerebellum, red nucleus and reticular formation. Bursts of activity recorded in the red nucleus in behaving monkeys, cats and turtles correlate closely with parameters of movement such as velocity and appear to represent simple motor programs. Burst discharges can also be elicited in the in vitro preparation. The goals of the proposed experiments are to examine the cellular and circuit mechanisms that contribute to the generation of burst discharges in the red nucleus, and by analogy, to motor program generation. Extracellular and intracellular recordings of red nucleus neurons in combination with ion substitution and application of activity-dependent dye will be performed to study the relative contributions of recurrent excitation and intrinsic membrane properties in the production of burst discharges. Mechanisms must also be present to modify movement commands so that they are adaptive to the environment. Mechanisms that might be used to adjust red nucleus motor output could include neurotransmitters or neuromodulators, such as GABA or serotonin. Studies of the fine control of burst discharges will be examined with extracellular recordings and iontophoresis of neurotransmitters and their antagonists. These studies of the cellular and circuit bases of synaptic integration underlying activity in the red nucleus will provide new data required to better understand the contribution of cerebellorubral synaptic pathways to adaptive motor control and to movement disorders, particularly Parkinson's disease.