MOLECULAR GENETICS OF DISEASE RESISTANCE IN ARABIDOPSIS

植物抗性的表达是植物对外来入侵的反应， 植物病原体是由植物的能力， 特异性地识别给定病原体。这个项目的总体目标是 研究项目是为了更好地了解植物病原体 识别和信号转导事件，导致表达 植物的抗病性。基因、分子和 生物化学方法将用于鉴定和表征植物 拟南芥中控制抗病性的基因 细菌病原体假单胞菌助理拟南芥RPS2病 抗性基因，其控制特异性P. 菌株，以前已分离和测序。然而，作用 该基因在病原体识别中的作用尚不清楚。主 本提案所述实验的目的是： 进一步表征RPS2及其功能，以及2）识别和 表征参与病原体识别的新植物基因座， 反抗的表现。将采用三种相辅相成的办法 来鉴定和表征与抗性相关的新基因座。第一 一种方法是识别与RPS2相互作用的细胞成分 基因产物将鉴定编码这些组分的基因， 利用反向遗传技术研究了它们在抗性中的作用。的 第二种方法涉及一个基因的遗传作图和分子克隆， 参与抗病性的新位点，其定义为部分 易感rps132突变体。第三种方法是识别新基因 通过分离和表征抑制因子参与耐药性， 明确定义的抗性突变体的增强剂。预计各国 这些策略将导致识别基因座编码 耦合病原体的信号转导机制的组成部分 识别抗病性的表达。识别 这些组件的特性将有助于我们 了解植物抗病性的分子基础。

The expression of plant resistance in response to attack by phytopathogenic organisms is triggered by the plant's ability to specifically recognize a given pathogen. The overall goal of this research project is to gain a better understanding of the plant-pathogen recognition and signal transduction events that lead to the expression of disease resistance in plants. A combination of genetic, molecular and biochemical approaches will be used to identify and characterize plant genes that control disease resistance in Arabidopsis thaliana to the bacterial pathogen Pseudomonas syringae. The A. thaliana RPS2 disease resistance gene, which governs recognition of specific P. syringae strains, has been previously isolated and sequenced. However, the role of this gene in pathogen recognition remains unclear. The primary objectives of the experiments described in this proposal are to I) further characterize RPS2 and it's function and 2) identify and characterize new plant loci that are involved in pathogen recognition and the expression of resistance. Three complementary approaches will be used to identify and characterize new loci involved in resistance. The first approach is to identify cellular components that interact with the RPS2 gene product. The genes encoding these components will be identified and their role in resistance studied using reverse genetic techniques. The second approach involves the genetic mapping and molecular cloning of a new locus involved in disease resistance that is defined by the partially susceptible rps132 mutant. The third approach is to identify new genes involved in resistance by isolating and characterizing suppressors and enhancers of well-defined resistance mutants. It is anticipated that these strategies will lead to the identification of loci encoding components of the signal transduction mechanism coupling pathogen recognition to the expression of disease resistance. The identification and characterization of these components will contribute to our understanding of the molecular basis of disease resistance in plants.