SOLID PHASE PLATE ASSAY (SPPA) FOR HIV1 GENOTYPING

描述：（改编自申请人摘要）这是一项I期研究 开发一种分子检测方法， 血浆和PBMC中的HIV-1亚型。该测定涉及基于RT-PCR的 与微孔板ELISA兼容的捕获杂交形式 法生物素标记的gag和env PCR产物将在 含有结合的寡核苷酸探针的微量滴定威尔斯孔， 比色反应代表gag不同序列的探针 和env基因将被用来区分不同的HIV-1毒株， M和O的组合。

DESCRIPTION: (Adapted From Applicant's Abstract) This is a Phase I study to develop a molecular assay for the genetic differentiation of the major HIV-1 subtypes in plasma and PBMCs. The assay involves a RT-PCR based capture hybridization format compatible with the microplate ELISA technique. Biotin-labeled gag and env PCR products will be captured on microtiter wells containing bound oligonucletide probes and detected by colorimetric reaction. Probes representing different sequences of the gag and env genes will be used to distinguish different strains of HIV-1 from both M and O groups.