NEW TRANSDERMAL VACCINE DELIVERY SYSTEM

拟议研究的目的是论证可行性。 一种新的透皮疫苗和/或药物输送方法，其结合了 抗原微胶囊化技术及新型电穿孔技术 方法。该方法将允许微球无痛地通过 松果体层(SC)进入有活力的表皮和真皮。 微胶囊提供了疫苗抗原的定时释放，它可以 减少所需的抗原剂量，它可以稳定疫苗， 从而延长其保质期。目前还没有开发出一种方法 用于微囊化抗原的经皮给药。这项研究 建议开发一种新的透皮给药方法 通过皮肤携带抗原的微球。该方法将具有 能够同时给药带电荷和不带电荷的微球。在……里面 第一阶段，微球经皮给药的新方法 将开发和评估含有金黄色葡萄球菌肠毒素A的产品 在体外和体内通过测量对 小鼠体内的肠毒素。在第二阶段，将对该方法进行进一步评估 运送其他抗原和/或蛋白质和原型装置将 都是设计好的。 拟议的商业应用：预计新的透皮贴剂 疫苗和/或药物输送系统将被设计为一次性 它将连接到便携式电压发生器以 注射疫苗或药物。新的系统将是无痛的， 价格低廉，卫生，并将在疫苗方面有巨大的应用 在第三世界国家送货。此外，该系统还可以广泛应用于 在定时和受控释放的情况下预防或治愈疾病 需要将药物转化为体液。

The objective of the proposed research is to demonstrate the feasibility of a new transdermal vaccine and/or drug delivery method which combines the technology of antigen microencapsulation and a novel electroporation method. The method will allow painless delivery of microspheres through the stratum comeum (SC) and into the viable epidermis and dermis. Microencapsulation provides timed release of vaccine antigen, it can reduce the dose of antigen required and it can stabilize the vaccine, thereby prolonging its shelf life. Currently there is no developed method for transdermal delivery of microencapsulated antigens. This research proposes the development of a new transdermal method for the delivery of antigen loaded microspheres through the skin. The method will have the capability of administering both charged and non-charged microspheres. In Phase I, the new method for transdermal delivery of microspheres containing Staphylococcal enterotoxin A will be developed and evaluated both in vitro and in vivo by measuring the immune response to the enteroxin in mice. In Phase II, the method will be further evaluated for the delivery of other antigens and/or proteins and a prototype device will designed. PROPOSED COMMERCIAL APPLICATION: It is envisaged that the new transdermal system for vaccine and/or drug delivery will be designed as a disposable "patch" which will be connected to a portable voltage generator to administer the vaccine or drug. The new system will be painless, inexpensive and sanitary and would have enormous applications for vaccine delivery in Third World countries. Also, the system can be widely used in disease prevention or cure where timed and controlled release of pharmaceuticals into body fluids is needed.