ABSORPTION OF ELLAGITANNINS FROM DIETARY SOURCES

描述：本项目的长期目标是确定 膳食来源中存在可水解鞣花单宁预防癌症， 人类 这个项目更直接的目的是评估a B s o 树莓中存在的可水解鞣花单宁的吸收和代谢， 草莓和核桃被人体消化系统吸收。 我们认为 可水解的鞣花单宁被人体血流吸收， 在乌藨子、草莓和核桃被食用后代谢。 我们 也相信这些鞣花单宁和代谢物可以被鉴定， 量化。 这样做的目的是鉴定生物活性 用于进一步细胞和动物化学预防的鞣花单宁和代谢物 问题研究 这项研究还将确定哪些饮食来源提供了 血液中生物活性鞣花单宁的最高浓度 流，这将有助于继续人类化学预防临床 审判 为了鉴定和定量吸收到细胞中的鞣花单宁， 乌藨子、草莓和核桃被食用后的血液流动， 将30名健康志愿者分成三个相等的组， 进入MUSC综合临床研究中心。 基线尿后 获得血清样本后，志愿者将被喂食已知量的 树莓、草莓或核桃在受控的条件下。 将采集尿液，并从每个患者中连续抽血 志愿者在24小时的研究期间。 已知的鞣花单宁将是 通过HPLC在血液和尿液中鉴定和定量。 未知 鞣花单宁和鞣花单宁代谢物将通过HPLC分离， 提交进行质谱（FAB）、NMR、UV和FR-IR分析， 可以进行结构确定。

DESCRIPTION: The long term goal of this project is to determine if hydrolyzable ellagitannins present in dietary sources prevent cancer in humans. The more immediate purpose of this project is to assess the a b s o r ption and metabolism of hydrolyzable ellagitannins present in raspberries, strawberries and walnuts by the human digestive system. We believe that the hydrolyzable ellagitannins are absorbed into the human blood stream and metabolized after raspberries, strawberries and walnuts are consumed. We also believe that these ellagitannins and metabolites can be identified and quantified. The purpose for doing this is to identify biologically active ellagitannins and metabolites for further cell and animal chemoprevention studies. This study will also identify which dietary source delivers the highest concentration of biologically active ellagitannins to the blood stream, which will be useful for continuing human chemoprevention clinical trials. In order to identify and quantify the ellagitannins absorbed into the blood stream after raspberries, strawberries, and walnuts are consumed, thirty healthy volunteers will be broken into three equal groups and admitted to the MUSC General Clinical Research Center. After baseline urine and serum samples are obtained, the volunteers will be fed a known quantity of either raspberries, strawberries or walnuts under controlled conditions. Urine will be collected and a serial blood draw will be taken from each volunteer over the 24-hour study period. Known ellagitannins will then be identified and quantified in the blood and urine by HPLC. Unknown ellagitannins and ellagitannin metabolites will be isolated by HPLC and submitted for Mass Spectrum (FAB), NMR, UV and FR-IR analysis so that structural determinations can be made.