RNA EDITING IN PLANT ORGANELLES

RNA编辑改变了预测的核或细胞器基因产物 许多生物，从原生动物到人类。这种独特的形式 使生物体在生产过程中具有独特的灵活性 来自DNA基因组的信使和结构RNA和蛋白质。为 一些动植物的细胞器，RNA编辑可以被视为一种 对原本不能表达或表达的基因的纠正机制 会对异常产品进行编码。RNA编辑有时也可用于 允许生产具有不同功能或性质的蛋白质 来自单一基因。在人类中，与脂质有关的基因的转录本 新陈代谢和神经系统功能属于基础阶段。 修改。碱基修饰RNA编辑的基本方面 将被发现是通过对C-U RNA编辑的研究发现的 被子植物。与发生RNA编辑的其他细胞器不同 可以通过刻意改变编辑位置来转化质体 分析催化和识别所需的顺式作用元件 用于修饰的碱基。突变筛选将识别核或 影响编辑位点识别和编辑的细胞器基因 效率。利用从变换分析中获得的信息， 底物RNA可以被设计成识别编辑的组成部分 与目标转录本相关的设备。拟议的研究 应加强对其他形式的RNA加工的理解 生物界，特别是tRNA、mRNA和rRNA的作用机制 修改和编辑。

RNA editing alters the predicted nuclear or organelle gene products from many organisms, ranging from protozoans to humans. This distinctive form of RNA processing gives organisms unique flexibility in producing messenger and structural RNAs and proteins from DNA genomes. For organelles of some plants and animals, RNA editing can be viewed as a correction mechanism for genes that otherwise could not be expressed or would encode aberrant products. RNA editing also sometimes serves to allow the production of proteins with diverse functions or properties from a single gene. In humans, transcripts of genes involved in lipid metabolism and nervous system function are among those that undergo base modifications. Fundamental aspects of base modification RNA editing will be uncovered by studies of C-to-U RNA editing in plastids of angiosperm plants. Unlike other organelles in which RNA editing occurs plastids can be transformed with deliberately altered editing sites to analyze the cis-acting elements necessary for catalysis and recognition of bases for modification. Mutant screens will identify nuclear or organelle genes which affect editing site recognition and editing efficiency. With information obtained from transformation analysis, substrate RNAs can be designed to identify components of the editing apparatus that associate with target transcripts. The proposed research should enhance understanding of other forms of RNA processing in the biological world, especially the mechanisms of tRNA, mRNA and rRNA modification and editing.