CHROMATIN STRUCTURE AND STABILITY IN THE SOLUTION STATE

描述：真核生物中的第一级DNA组织存在 在核小体中，核小体由大约200个核苷酸包裹的DNA组成 围绕着组蛋白八聚体。核小体被认为是最多的 染色体结构的基本元素。其他公司成立为法团 蛋白质进入寡核小体阵列可产生特定的染色质 具有特定核功能的结构，如转录 活动。 拟议研究的总体目标是确定和 描述核小体阵列的结构和功能状态 并了解组蛋白、连接器组蛋白和 转录因子在指导和维持更高秩序中的作用 核小体阵列折叠和自身结合所产生的染色质结构 协会。提出了三项具体任务来解决 假设核小体的固有构象动力学 阵列有助于形成30 nm和更高的染色质纤维 染色体区域，而这种折叠为RNA提供了一种屏障 可以被其他蛋白质如H1修饰的聚合酶 转录活动的特定状态。这些研究是预期的 为实现装配目标提供必要的基准 体外转录激活和抑制染色质状态的研究 纯组件。

DESCRIPTION: The first level of DNA organization in eukaryotes resides in the nucleosome, which consists of approximately 200 bp of DNA wrapped around the histone octamer. The nucleosome is regarded as the most fundamental element of chromosomal structure. Incorporation of other proteins into oligonucleosomal arrays gives rise to specific chromatin structures capable of specific nuclear functions such as transcriptional activity. The general goals of the proposed research are to identify and characterize the structural and functional states of nucleosomal arrays and to understand the role of histones, linker histones, and transcription factors in directing and maintaining higher order chromatin structures brought about by nucleosome array folding and self- association. Three specific tasks are proposed to address the hypothesis that the intrinsic conformational dynamics of nucleosomal arrays contribute to formation of the 30 nm chromatin fiber and higher chromosomal domains, and that folding provides a barrier to RNA polymerases that can be modified by other proteins such as H1 to achieve specific states of transcriptional activity. These studies are expected to provide the baseline necessary to attain the goal of assembling transcriptionally active and repressed chromatin states in vitro from pure components.