QUANTITATION OF CARDIAC MYOCYTE CONTRACTILE DYNAMICS

许多旨在理解生理学的基本问题 特别是心肌的病理生理学 使用分离的心肌细胞在细胞水平上解决，或 肌细胞 该应用程序的主要目的是开发简单的 和经济的数字成像工具， 社区可以用来记录肌细胞的生物力学特性 在肌肉收缩时。 该项目将作为三个 具体开发目标：（1）高速数字图像 采集收缩的心肌细胞以使其可视化，（2） 基于软件的肌细胞长度记录，以执行实时 分析数字图像数据，以动态表征、显示和 在线保存心肌细胞长度数据，以及（3）肌节 长度记录扩展了高速图像采集的方法 和数值分析来实时量化肌节长度 在肌肉收缩时。这些方法从根本上改善了 现有技术在功能能力和简单性方面 使用. 在商业化形式中，所设想的仪器系统是 也是经济的，因此可供更多感兴趣的实验室使用。 在细胞水平上研究心肌问题。

Many of the fundamental questions aimed at understanding the physiology and, particularly, the pathophysiology of the myocardium are now being addressed at the cellular level using isolated cardiac muscle cells, or myocytes. The principal aim of this application is to develop simple and economical digital imaging tools that the cardiac muscle research community can use to record the biomechanical properties of myocytes during muscle contraction. The project will be undertaken as three Specific Aims for the development of: (1) High-Rate Digital Image Acquisition for visualization the contracting cardiac myocyte, (2) Software-Based Myocyte Cell Length Recording to perform real-time analysis of digital image data to dynamically characterize, display and save the cardiac myocyte cell length data on-line, and (3) Sarcomere Length Recording expands on the methods of high-rate image acquisition and numerical analysis to quantify the sarcomere length in real-time during muscle contraction. These methods fundamentally improve on existing techniques in terms of functional capability and simplicity of use. In commercialized form the instrumentation systems envisioned are also economical and thus accessible to many more laboratories interested in pursuing cardiac muscle research questions at the cellular level.