SPECIFIC BINDING PROTEINS FOR HUMAN IMMUNOGLOBULIN G

这项建议的重点是开发人免疫球蛋白亚类特异性 细菌结合蛋白。这些蛋白质将对 量化特定的免疫球蛋白亚类，研究亚类对 不同的抗原和免疫方案，确定亚类 生物样品中的分布及单个人免疫球蛋白的纯化 子类。重组细菌免疫球蛋白亚类试剂的研制 根据这项提议，将为现有的反 人类免疫球蛋白亚类单抗，已被证明具有 世界卫生组织最近赞助的研究的严重局限性 组织。在第一阶段的研究中，重组细菌结合 针对人IgG3的蛋白质被证明是有效的 人免疫球蛋白3的灵敏和特异的检测/定量 化验格式。候选结合蛋白专用于每一个 还鉴定了其他个别的免疫球蛋白亚类。在第二阶段， 重组IgG1、1gG2和IgG4特异性人免疫球蛋白亚类结合 将生产和测试用于诊断的蛋白质 免疫化学程序和亲和纯化方法。井 成熟的基因工程技术将被用来产生 具有所需功能的重组亚类结合蛋白 特点。第二阶段研究将提供人类免疫球蛋白亚类特异性 用于基础研究、免疫诊断和生物处理的试剂。 建议的商业应用： 可以选择性地与特定人结合的细菌结合蛋白 免疫球蛋白亚类将为研究和诊断提供关键试剂 免疫分析。可以检测出猪瘟病毒中免疫球蛋白亚类的细菌蛋白 类似于葡萄球菌蛋白A或链球菌蛋白G 也将在大规模的商业应用中发挥重要作用 免疫球蛋白亚类抗体的纯化。

The focus of this proposal is to develop human IgG subclass specific bacterial binding proteins. These proteins will be of value for quantifying specific IgG subclasses, studying subclass responses to different antigens and immunization protocols, determining subclass distribution in biological samples and purifying individual human IgG subclasses. The recombinant bacterial IgG subclass reagents developed under this proposal will provide a reliable alternative to existing anti- human IgG subclass monoclonal antibodies which have been shown to have serious limitations in recent studies sponsored by the World Health Organization. During Phase I studies, a recombinant bacterial binding protein specific for human IgG3 was shown to be effective for the sensitive and specific detection/quantitation of human IgG3 in a variety of assay formats. Candidate binding proteins specific for each of the other individual IgG subclasses were also identified. In Phase II, recombinant IgG1, 1gG2 and IgG4 specific human IgG subclass binding proteins will be produced and tested for utility in diagnostic immunochemical procedures and affinity purification methods. Well established genetic engineering techniques will be used to generate recombinant subclass binding proteins with the desired functional characteristics. Phase II studies will provide human IgG subclass specific reagents for use in basic research, immunodiagnostics and bioprocessing. Proposed commercial applications: Bacterial binding proteins that could bind selectively to specific human IgG subclasses will provide key reagents for research and diagnostic immunoassays. Bacterial proteins that could detect IgG subclasses in an analogous fashion to staphylococcal protein A or streptococcal protein G would also have significant commercial applications for large scale purification of IgG subclass antibodies.