GENOMIC ANALYSIS OF LAMBDOID, TB & OTHER BACTERIOPHAGES

Dsdna噬菌体是进化生物中数量最多的类群。 在生物圈里。目前可从基因组测序中获得的信息 揭示了对它们进化的重要见解， 并提供了充分的理由相信，适量的额外 全基因组的序列测定将大大增加我们的 了解噬菌体种群的遗传结构及其 进化机制和历史。我们建议确定 九个噬菌体基因组的序列，其中三个是Lambdoid家族， 四个来自分枝杆菌噬菌体，另外两个。我们将分析和 比较这些序列以阐明它们的遗传组织并使 关于噬菌体的进化推论。 除了为噬菌体提供进化信息外， 本项目有望为噬菌体的基础研究做出贡献。 通过提供关于基因组织和功能的比较数据来进行生物学 以及通过使新的噬菌体可用于详细的 实验。此外，预计来自该项目的序列数据 协助利用噬菌体分析和对抗人类疾病 由宿主引起的，特别是包括肺结核。

The dsDNA bacteriophages are the most numerous taxon of evolving organisms in the biosphere. Information currently available from genomic sequencing of these organisms has revealed important insights into their evolution, and has given good reason to believe that a modest amount of additional sequence determination of whole genomes will substantially augment our understanding of the genetic structures of phage populations and of their evolutionary mechanisms and history. We propose to determine the sequences of nine bacteriophage genomes, three of the lambdoid family, four from the mycobacteriophages, and two others. We will analyze and compare the sequences to elucidate their genetic organizations and to make evolutionary inferences about the phages. In addition to providing evolutionary information for the bacteriophages, this project is expected to contribute to basic research on bacteriophage biology by providing comparative data about gene organization and function as well as by making new bacteriophages available for detailed experimentation. In addition, sequence data from this project is expected to assist in using bacteriophages to analyze and counteract human diseases caused by their hosts, specifically including tuberculosis.