GENOMIC ANALYSIS OF LAMBDOID, TB & OTHER BACTERIOPHAGES

双链DNA噬菌体是进化生物体中数量最多的类群 in the biosphere生物圈. 目前可从基因组测序获得的信息 揭示了它们进化的重要见解， 并给出了很好的理由相信， 整个基因组的序列测定将大大增强我们的 了解噬菌体种群的遗传结构及其 进化机制和历史。 我们建议确定 九个噬菌体基因组的序列，三个噬菌体家族， 四个来自分枝杆菌噬菌体，另外两个。 我们将分析和 比较序列以阐明它们的遗传组织， 进化的推论。 除了为噬菌体提供进化信息外， 该项目有望为噬菌体的基础研究做出贡献 通过提供有关基因组织和功能的比较数据， 以及通过制造新的噬菌体， 实验 此外，预计该项目的序列数据 帮助使用噬菌体来分析和对抗人类疾病 由其宿主引起，特别是包括结核病。

The dsDNA bacteriophages are the most numerous taxon of evolving organisms in the biosphere. Information currently available from genomic sequencing of these organisms has revealed important insights into their evolution, and has given good reason to believe that a modest amount of additional sequence determination of whole genomes will substantially augment our understanding of the genetic structures of phage populations and of their evolutionary mechanisms and history. We propose to determine the sequences of nine bacteriophage genomes, three of the lambdoid family, four from the mycobacteriophages, and two others. We will analyze and compare the sequences to elucidate their genetic organizations and to make evolutionary inferences about the phages. In addition to providing evolutionary information for the bacteriophages, this project is expected to contribute to basic research on bacteriophage biology by providing comparative data about gene organization and function as well as by making new bacteriophages available for detailed experimentation. In addition, sequence data from this project is expected to assist in using bacteriophages to analyze and counteract human diseases caused by their hosts, specifically including tuberculosis.