TISSUE DIELECTRIC CHANGES DUE TO HYPERTHERMIA

描述：各种形式的癌症治疗，放射，细胞毒性药物， 体温过高等，都有一个共同的目标 我们的目标是根除 癌细胞 因此，当在实验室中测试疗法时， 或应用于临床，他们根除癌症的能力 细胞需要评估。 通常，端点，例如 使用肿瘤生长延迟或肿瘤消退，或者如果是中间状态， 如果需要信息，则必须获取侵入性组织样本。 近日 已经确定，细胞主要对所有形式的 以两种方式治疗有害刺激;通过程序性细胞死亡 （凋亡）和/或通过坏死。 本提案的目的是测试电阻抗的能力 光谱（EIS）检测和识别这两种形式的细胞反应 通过在生长的固体人类异种移植物中进行非侵入性体内测量 裸鼠 使用或不使用维拉帕米的高温治疗， 用于诱导不同形式的细胞反应。 然后是电气 肿瘤的阻抗测量将在 肿瘤生长延迟的治疗和表现的应用。 这些 EIS测量的目的是监测实时蜂窝级响应 肿瘤在此期间 本研究将验证以下假设：1. 有可能实现长期、 可靠、可重复、无创、体内电阻抗 组织电特性的光谱（EIS）测量。 2. EIS能够检测和表征长期， 高血压诱导的坏死反应序列在其发生期间， 以及区分坏死组织中不同特征的能力 不同的治疗引起的反应， 肿瘤生长延迟。 3. EIS能够检测和表征 具有高凋亡的实体瘤内的凋亡细胞反应序列 指数，使用非侵入性，在体内测量。 进展及初步 数据证明了测量细胞水平变化的可行性 EIS。

DESCRIPTION: Cancer therapies of all forms, radiation, cytotoxic drugs, hyperthermia, etc., have one goal in common. That goal is to eradicate cancer cells. Therefore, when therapies are being tested in the laboratory or applied in the clinic, the ability to which they eradicate the cancer cells needs to be evaluated. Typically, endpoints such as tumor-growth-delay or tumor regression are used, or if intermediate information is desired, invasive tissue samples must be taken. Recently, it has been determined that cells predominantly respond to all forms of treatment of deleterious stimuli in two ways; via programmed cell death (apoptosis) and/or via necrosis. The aim of this proposal is to test the capability of Electrical Impedance Spectroscopy (EIS) to detect and identify these two forms of cell response via non-invasive, in vivo measurements in solid human xenografts grown in nude mice. Treatment with hyperthermia, with and without verapamil, will be used to induce the different forms of cell response. Then electrical impedance measurements of the tumors will be made during the period between application of the treatment and manifestation of tumor-growth-delay. These EIS measurements will aim to monitor the real-time cellular-level response of the tumor during this period. The work will test the hypotheses: 1. It is possible to make long-term, reliable, repeatable, non-invasive, in vivo Electrical Impedance Spectroscopy (EIS) measurements of the electrical properties of tissues. 2. EIS is capable of detecting and characterizing long-term, hyperthermia-induced, necrotic response sequences during their occurrence, and of discriminating between different characteristics in the necrotic responses caused by different treatments which result in different tumor-growth-delays. 3. EIS is capable of detecting and characterizing the apoptotic cell response sequence within a solid tumor with high apoptotic index, using non-invasive, in vivo measurements. Progress and preliminary data demonstrate the feasibility of measuring cellular-level change with EIS.