NEUROGENETICS OF DROSOPHILA REPRODUCTION

果蝇的生殖神经遗传学涉及一系列不断扩大的 行为和神经突变以及分子操纵 涉及影响果蝇性别特异性特征的因素， 神经系统为了扩展半古典求爱的分析 基因，钙通道多肽和 将剖析核酸结合蛋白。对于前者， 这两个基因，杂音，实验包括分子- 求偶歌缺陷和视觉系统缺陷的遗传分析， 好吧（后者是由某些cac位点突变引起的）。 转录后变异在通道质量中的意义 将进行评估，包括分子操作和行为 涉及cac s主要的假定RNA编辑事件的生物测定 产品非瞬时A基因也可以突变， 唱歌和视觉异常非A空间的意义 表达将被解剖，主要是在一个小说方面 与转基因相关的求偶嗡嗡声异常 正常等位基因的操作。非A和假定的 它编码的RNA结合蛋白可能与cac相互作用， 部分通过确定体内是否存在 基因工程nonA突变影响转录后加工 Ca ~（2+）通道RNA cac行为和分子遗传学研究 暗示了求偶歌曲在进化上的一种分歧： 调节由钙离子引起的神经元输出模式， 信道变化;这将通过分析间， cac的特定亲属和cac中分子变体的生物测定， 转基因雄性性别决定的遗传学将进一步 结合行为和信息素的求偶研究 分析无果和double-ext突变体;实验包括 求偶-嗡嗡声异常的分子神经发生学研究 和性别特异性运动行为的分析。在某些情况下， 新的方向，女性接受性的遗传学将进行分析 通过对一种新突变体的神经生物学和分子学研究， 生物钟的可能重置，由条件反射引起， 求偶刺激，将进行调查，看看是否可以平行 这一领域在哺乳动物身上的新发现吸引了我。-- 从表面上看，生殖似乎具有广泛的意义。 这些现象的变异，在生物体中由遗传引起 一直到人类，都在被越来越多的人欣赏，分析， 实验性地制造。这表明，神经遗传 以及分子神经生物学的发现和原理， 从果蝇求偶和交配的研究中分离出来， 有助于设计和解释这方面的调查 这将涉及各种不同的生物。

Reproductive neurogenetics in Drosophila involves a widening array of behavioral and neural mutants as well as molecular manipulations involving factors that influence sex-specific features of the fly's nervous system. To extend the analysis of semi-classical courtship genes, the roles played by calcium-channel polypeptides and nucleic-acid binding proteins will be dissected. For the former of these two genes, cacophony, the experiments include molecular- genetic analysis of courtship-song defects and visual-system ones as well (the latter being caused by certain of the cac-locus mutations). The significance of posttranscriptional variation in channel quality will be assessed, including molecular manipulations and behavioral bioassays of putative RNA editing events involving cac s primary product. The no-on-transient-A gene also can be mutated to cause singing and visual abnormalities. The significance of nonA's spatial expression will be dissected, principally in terms of a novel abnormality of courtship-humming sounds associated with transgene manipulations of the normal allele. How nonA and the putative RNA-binding protein it encodes may interact with cac will be determined, in part by determining whether in vivo an and engineered nonA mutations affect post-transcriptional processing of the Ca2+-channel RNA cac's behavioral and molecular-genetics suggests one way that courtship songs diverge evolutionarily: by modulations of patterned neuronal outputs caused by calcium- channel variations; this will be investigated by analyzing inter- specific relatives of cac and bioassays of the molecular variants in transgenic males. The genetics of sex determination will be further merged with courtship studies by behavioral and pheromonal analysis of fruitless and doublesex mutants; the experiments include molecular neurogenetic dissection of courtship-hum abnormalities and analysis of sex-specific locomotor behavior. To move in certain new directions, the genetics of female receptivity will be analyzed by neurobiological and molecular studies of a new mutant; also, possible resets of a biological clock, induced by conditioned courtship stimuli, will be investigated to see if parallels can be drawn to a novel finding in this area from mammals. -- Reproduction seems on the face of it to have broad significance. Variations of these phenomena, caused genetically in organisms ranging up to humans, are being increasingly appreciated, analyzed, and manufactured experimentally. It is suggested that neurogenetic and molecular neurobiological findings and principles, stemming in part from studies of Drosophila courtship and mating, may contribute to the design and interpretation of investigations in this area, which would involve a variety of different organisms.