YEAST NUCLEOSIDE DIPHOSPHATE KINASES

核苷二磷酸(EC2.7.4.6.)(NDP激酶)是必需的 核糖核苷二磷酸和脱氧核苷二磷酸的磷酸化 它们相应的三磷酸盐，从而为 DNA、RNA和其他细胞成分的生物合成。近期 研究表明，NDP激酶基因的功能也参与了 发育、致癌及其与GTP结合的相互作用 各种生物体中的蛋白质家族。尽管这些结果是 耐人寻味的是，大多数此类研究仍处于初步阶段。我们建议使用 以发芽酵母酿酒酵母为模型研究 NDP激酶在细胞内的基因功能。这样做的最初目标是 建议研究NDP和DTMP的相互作用 (和其他核苷酸激酶)，以描述它们之间的相互作用 领域(S)和揭示这样的生物学意义 互动。第二个目标是确定一组迄今未知的 与NDP激酶配位的成分，从而阐明Ndk1‘S 推定的相互关联的路径。第三个目标是识别和描述 推测的第二个NDP激酶(暂定为NDP激酶-II) 放在酵母里。我们期望对酵母NDK1基因和 它的相关蛋白质成分(S)将导致对 酵母中的几个重要生物学功能；扩展到 相对定义明确的酵母菌，可能会让我们更好地 了解NDP激酶在其他真核生物中的不同功能。

Nucleoside diphosphate kinase (EC 2.7.4.6.) (NDP kinase) is required for the phosphorylation of ribo- and deoxyribo- nucleoside diphosphates to their corresponding triphosphates, thereby providing building blocks for the biosynthesis of DNA, RNA and other cellular components. Recent studies suggest that the NDP kinase gene functions are also involved in development, oncogenesis, and in interaction with the GTP-binding protein family in various organisms. Although these results are intriguing, most such studies are still preliminary. We propose to use the budding yeast Saccharomyces cerevisiae as a model to investigate the gene functions of NDP kinase within the cell. The initial goal of this proposal is to investigate the interaction of NDP kinase and dTMP kinase (and other nucleotide kinases) in order to delineate their interacting domain(s) and to uncover the biological significance of such interaction. The second goal is to identify an array of thus far unknown components which coordinate with NDP kinase, so as to elucidate NDK1's putative linked pathways. The third goal is to identify and characterize a putative second NDP kinase (tentatively designated as NDP kinase-II) in yeast. We expect that the investigation of the yeast NDK1 gene and its related protein component(s) will lead to an understanding of several important biological functions in yeast; extension beyond the relatively well-defined yeast organisms, may allow us to better understand the diverse functions of NDP kinase in other eukaryotes.