ECHOCARDIOGRAPHIC STUDY OF THE CORONARY MICROVASCULATURE

描述：（改编自研究者摘要） 申请补助金的目的是研究 冠状动脉微血管 调查人员希望能巩固观察结果 心肌造影剂中使用的微泡如何与 内皮细胞和内皮细胞基质，因此可以作为 正常和破裂的内皮细胞。 其次，调查人员还 计划应用同样的技术，心肌造影超声心动图， 血管功能的离体和体内模型。 这个项目的最终目标 建议是开发技术，使调查人员获得 临床前内皮功能/功能障碍的病理生理学见解 和血管生成。 虽然缺血性心脏病 对冠状动脉微血管系统的许多影响，本研究者将 考察两个方面。 首先，根据她先前的观察， 涂覆的、充满空气的微泡（其用于心肌造影剂 超声心动图）与内皮细胞相互作用，研究者将 试图量化这种相互作用的具体决定因素，希望 它是血管内皮功能的标志物。 二是 研究者将研究微血管对缺血的反应， 血管生成和侧支血管的发展。 这两个过程 很难直接在体内研究， 心肌造影超声心动图可以实现对心肌的真实的时间检查， 冠状动脉微血管 因此，这项建议的两个具体目标是： 1. 微泡-内皮细胞作用的部位和机制 使用细胞培养和光学成像技术的相互作用， 这些发现可以通过超声心动图研究内皮功能， 体内和体内动物模型;和2. 开发一种新的超声心动图 测量微血管血容量的方法，然后将其用于 一系列非侵入性检查的分布，自然史， 特异性成纤维细胞生长诱导的侧支血管血流储备 因子

DESCRIPTION: (Adapted from the Investigator's Abstract) The work in this grant application attempts to study the structure and function of the coronary microvasculature. The investigator hopes to solidify observations of how microbubbles used in myocardial contrast echo interact with endothelial cells and endothelial cell matrix and thus may act as markers of normal and disrupted endothelial cell. Secondly, the investigator also plans to apply the same technology, myocardial contrast echocardiography, to ex vivo and in vivo models of vascular function. The ultimate goal of this proposal is to develop techniques which will allow the investigator to gain pathophysiologic insights into pre-clinical endothelial function/dysfunction and angiogenesis in the intact animal. Although ischemic heart disease has many effects on the coronary microvasculature, this investigator will examine two aspects. First, based on her earlier observation that albumin coated, air-filled microbubbles (which are used in myocardial contrast echocardiography) interact with endothelial cells and the investigator will attempt to quantify the specific determinants of this interaction in a hope that it is a marker of vascular endothelial function. Secondly, the investigator will study the microvascular response to ischemia in the form of angiogenesis and collateral vessel development. Both of these processes have been difficult to study directly in vivo and the development of myocardial contrast echocardiography may enable real time examination of the coronary microvasculature. Thus, the two specific aims of this proposal are 1. to characterize sites and mechanism of microbubble-endothelial cell interaction using cell culture and optical imaging techniques and to use these findings to echocardiographically study endothelial function in ex vivo and in vivo animal models; and 2. to develop a new echocardiographic approach to measuring microvascular blood volume and then to use it for serial non-invasive examination of the distribution, natural history, and flow reserve of collateral vessels induced by specific fibroblast growth factor.