POLYMER MEMBRANE ION/POLYION SENSORS--NEW FRONTIERS

描述：(申请人摘要)继续发展、学习和 新型离子和多离子敏感聚合物的生物分析应用 提出了一种基于膜的电化学传感器。在研究过程中 下一个项目期将建立在关于 良好的多离子(例如，肝素，鱼精蛋白， 合成多肽)制成增塑聚合物薄膜，其中包含 适当的亲油性离子交换剂以及选择性相互作用 阴离子与给定的金属离子-配体络合物设计传感器，将 能够直接检测复合体中具有生物医学意义的重要物种 样本，包括全血。这些努力将包括基本的 和应用研究，特别强调扩大范围 可通过简单相界面电位法(EMF)监测的物种 跨聚合物膜/溶液界面的测量。的具体目标 多离子传感器的工作包括：a)使用合成的 聚阳离子/聚阴离子多肽作为测试化合物来确定 聚离子结构/电荷密度影响聚离子热力学 提取到适当配方的薄膜中；b)设计高度具体的 用优化的人工合成多离子多肽测定蛋白酶活性 以及c)调查制备简化材料的能力 使用亲脂性银离子/杯芳烃的固态型多离子传感器 复合体作为额外的薄膜添加剂。超越对多离子的进一步研究 金属卟啉作为膜活性物质用于传感膜的研究 将继续开发新的阴离子选择性传感器的组件， 特别注重理解超级能人的行为经常 用这样的体系观察，并在获得分析上有用的阴离子 通过改变金属中心离子和添加合适的 聚合物敏感膜内的亲脂性阴离子或阳离子位置。 正在努力使用分子/离子印迹原理来创造 更多选择性离子/多离子传感器将通过采用新的 光聚合甲基丙烯酸酯薄膜的开发和详细研究 在最近的赠款期间。预计这项研究 计划将继续为生物医学社区提供广泛的 新的和/或改进的化学传感器以及基于传感器的新方法 它将立即作为基础研究和内部研究的工具 现代临床化学仪器。

DESCRIPTION: (applicant's abstract) The continued development, study, and bioanalytical applications of novel ion and polyion sensitive polymer membrane based electrochemical sensors are proposed. Research during the next project period will build upon recent discoveries regarding the favorable extraction thermodynamics of polyions (e.g., heparin, protamine, synthetic polypeptides) into plasticized polymeric films containing appropriate lipophilic ion-exchangers as well as selective interactions of anions with given metal ion-ligand complexes to devise sensors that will enable the direct detection of biomedically important species in complex samples, including whole blood. These efforts will include both fundamental and applied studies, with particular emphasis on expanding the range of species that can be monitored via simple phase boundary potentiometric (EMF) measurements across polymeric film/solution interfaces. Specific goals for the polyion sensor efforts include: a) using synthetic polycationic/polyanionic peptides as test compounds to determine how the polyion structure/charge density affects the thermodynamics of polyion extraction into appropriately formulated films; b) designing highly specific assays for protease enzymes using optimized synthetic polyion peptides as substrates; and c) investigating the ability to prepare simplified solid-state type polyion sensors using lipophilic silver ion/calixarene complexes as additional film additives. Beyond further studies of polyion sensing films, research on the use of metalloporphyrins as membrane active components in the development of new anion selective sensors will continue, specifically focusing on understanding the super-Nernstian behavior often observed with such systems, and on achieving analytically useful anion selectivities via changes in the metal center ion and addition of suitable lipophilic anion or cationic sites within the polymeric sensing films. Ongoing efforts to use the principles of molecular/ion imprinting to create more selective ion/polyion sensors will be accelerated by employing new photopolymerizable decyl methacrylate films, developed and studied in detail during the most recent grant period. It is anticipated that this research program will continue to provide the biomedical community with a wide array of new and/or improved chemical sensors as well novel sensor-based methods that will have immediate applications as tools for basic research and within modern clinical chemistry instrumentation.