SPECIFICITY STUDIES OF HIV AND HTLV PROTEASES

描述：(改编自申请人的摘要)建议书的短期内容 目的是为了弄清耐药变异体作用的分子基础。 人类免疫缺陷病毒1型(HIV-1)蛋白水解酶的结构和功能比较 HIV-1和Rous肉瘤病毒蛋白水解酶突变体的活性。这个 建议的特异性研究将是：1)涵盖更广泛的寡肽 为了更全面地描述耐药HIV-1的底物 蛋白水解酶突变体；以及2)促进对 相关的人T细胞白血病病毒1型的特异性 蛋白酶。长期目标是预测新的蛋白水解酶抑制剂和 克服抗药性问题的治疗策略。

Description: (Adapted from the Applicant's Abstract) The proposal's short-term aim is to eludicate the molecular basis for the action of resistant variants of Human Immunodeficiency Virus 1 (HIV-1) protease by comparing the structures and activities of selected mutants of HIV-1 and Rous sarcoma virus proteases. The specificity studies proposed will be: 1) to cover a broader set of oligopeptide substrates in order to more fully characterize the inhibitor-resistant HIV-1 protease mutants; and 2) promote new studies on the molecular basis for the specificity of the related human T-cell leukemia virus type 1 (HTLV-1) protease. The long-term aim is to predict new protease inhibitors and therapeutic strategies to overcome the problem of drug-resistance.