ASSEMBLY OF AN AMINOACYLATING RIBONUCLEOPROTEIN

一种能够使RNA氨酰化的核糖核蛋白(RNP)催化剂将 被组装起来。该RNP将基于丙氨酰-tRNA的截断形式 合成酶，合成氨基酰-AMP，但缺乏RNA识别 域。因此，将采用体外选择的方法来获得 一种合适的RNA适配子，将重建细胞的能力 与RNA底物结合的催化剂。氨酰化反应的效率 将对合成的RNP进行研究，并将RNA-蛋白质接口 在复合体中的存在将被用生化来表征 技巧。有人提出，核糖核蛋白是常见的 在进化过程中连接RNA和蛋白质的催化剂- 主宰了世界，很可能合成酶看起来非常 由于它们在蛋白质中的重要作用，所以在这种转变的早期 综合。因此，氨基酰化RNP的组装将提供 合成酶的可能进化前体的证据 含有RNA组分。

A ribonucleoprotein (RNP) catalyst capable of RNA aminoacylation will be assembled. This RNP will be based on a truncated form of alanyl-tRNA synthetase which synthesizes aminoacyl-AMP, but lacks an RNA recognition domain. Hence, in vitro selection methods will be employed to obtain a suitable RNA aptamer that will re-establish the ability of the catalyst to bind an RNA substrate. The aminoacylation efficiency for the resultant RNP will be investigated and the RNA-protein interfaces present within the complex will be characterized using biochemical techniques. It has been proposed that ribonucleoproteins were common catalysts during the period of evolution bridging RNA and protein- dominated worlds, and it is likely that the synthetases appeared very early in this transition because of their important role in protein synthesis. Thus, the assembly of an aminoacylating RNP would provide evidence for possible evolutionary precursors to the synthetases that contained RNA components.