ANTIBODY BASED METHODS FOR 2,3,7,8-TCDD ANALYSIS

描述：(改编自申请人的摘要)多氯化 二苯并对二恶英和二苯并呋喃(PCDD/FS)具有重要意义 环境污染物。对其环境进行全面评估 影响是困难的，因为多氯二苯并二恶英是210种可能的混合物 氯化异构体。此外，2，3，7，8-取代的分析 有毒的同系物多氯二苯并二恶英目前需要特异性、敏感性和 高分辨率GC/MS的费用基于抗体的分析方法有 展示了对高通量筛选和定量筛选的实用性 环境样品的分析。然而，以前的PCDD/F免疫分析 发展项目并不是特别成功。有一个 对敏感的多氯二苯并二恶英/F明确的科学、商业和经济需求 有毒同系物特异性免疫分析。这个项目的目标是 开发基于实验室的免疫分析方法，可用于 大大降低了多氯二苯并二恶英/氟有毒同系物分析的总体成本。 第一阶段的目标是证明他们可以开发出特定的和 敏感的2，3，7，8-TetraCDD导向抗体。第一阶段演示了这一点 目标与开发毒性当量物(TEQ)导向的多克隆 基于血清的检测和灵敏的2，3，7，8-TetraCDD特异性单抗 基于抗体的化验。第二阶段的研究将发展全套 所需的PCDD/F同类特定单抗和竞争对手，证明是商业的 通过市场引入特异性免疫分析的可行性 产品并启动免疫亲和试剂和 飞秒血象敏感分析。 建议的商业应用：不可用

DESCRIPTION: (Adapted from the applicant's abstract) Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) are significant environmental pollutants. Comprehensive assessment of their environmental impact is difficult because the PCDD/Fs are a mixture of 210 possible chlorinated isomers. Moreover, the analysis of the 2,3,7,8-substituted toxic congener PCDD/Fs currently requires the specificity, sensitivity and expense of high resolution GC/MS. Antibody based analytical methods have demonstrated utility for both high throughput screening and quantitative analysis of environmental samples. However, previous PCDD/F immunoassay development programs have not been particularly successful. There is a clear scientific, commercial and economic need for a sensitive PCDD/F toxic congener specific immunoassays. The goal of this project is to develop laboratory based immunoanalytical methods that can be used to substantially reduce the overall cost of PCDD/F toxic congener analysis. The goal of Phase I was to prove that they could develop specific and sensitive 2,3,7,8-TetraCDD directed antibodies. Phase I demonstrated this goal with the development of a Toxic Equivalents (TEQ) directed polyclonal serum based assay and a sensitive, 2,3,7,8-TetraCDD specific monoclonal antibody based assay. The Phase II studies will develop the full set of required PCDD/F congener specific MAbs and competitors, prove commercial feasibility through the market introduction of specific immunoassay products and initiate the development of immunoaffinity reagents and femtogram sensitive assays. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE