IDENTIFICATION OF MAMMALIAN HISTIDINE KINASES

细胞用来传输信号的机制 细胞外环境对于理解正常和 异常的细胞生长。蛋白质的磷酸化涉及错综复杂。 在传输由许多不同类型的细胞外启动的信号时 刺激物。真核细胞信号转导通路的研究主要集中在 丝氨酸、苏氨酸和酪氨酸磷酸化的作用 蛋白质。从许多实验室收集的数据表明， 磷酸组氨酸是哺乳动物细胞蛋白的重要组成部分。 组氨酸激酶和磷酸酶与哺乳动物有关 几个体外研究中的信号通路。最近，瞬时蛋白 组氨酸磷酸化已在体内被证明，作为对 凝血酶或胶原刺激启动的信号转导通路 血小板。组氨酸的分子后果评价 哺乳动物信号的磷酸化需要鉴定 组氨酸激酶。本项目的重点是生物化学。 哺乳动物组氨酸激酶的纯化及其分子克隆 酵素。这些研究有可能为以下领域带来新的曙光 真核信号转导。

Knowledge of the mechanisms cells use to transmit signals from the extracellular environment is crucial in understanding normal as well as aberrant cellular growth. Protein phosphorylation is intricately involved in transmitting signals initiated by many different types of extracellular stimuli. Studies of eukaryotic signaling pathways have focused on the contributions of serine, threonine and tyrosine phosphorylation of proteins. Data gathered from many laboratories indicate that phosphohistidine is a significant component of mammalian cellular protein. Histidine kinases and phosphatases have been implicated in mammalian signaling pathways in several in vitro studies. Recently, transient protein histidine phosphorylation has been demonstrated in vivo, in response to signaling pathways initiated by thrombin or collagen stimulation of human platelets. Appreciation of the molecular consequences of histidine phosphorylation for mammalian signaling requires identification of histidine kinases. The focus of this project is the biochemical purification of mammalian histidine kinases and molecular cloning of these enzymes. These studies have the potential to shed new light on the field of eukaryotic signal transduction.