NOVEL HIGHTHROUGHOUT SCREENING ASSAY

描述：（改编自申请人的摘要）本SBIR提案的目的 目的是开发一种新的、快速的、均相的高通量筛选方法 其能够检测抗体/抗原之间的亲和相互作用， 受体/配体和酶/底物。高通量筛选（HTS）试验 基于这项新技术将在制药和 生物技术组合药物发现计划。具体目标将是 开发用于测定的新型试剂，优化测定条件， 开发用于执行分析的仪器。HTS测定结合了 使用特别配制的光合膜和荧光检测。 拟议的工作将集中在开发一种HTS检测方法，用于鉴定 参与信号转导的蛋白酪氨酸激酶抑制剂。一个 将开发一种检测方法来鉴定src（一种致癌蛋白）的抑制剂 酪氨酸激酶在某些癌细胞中过度表达。 拟定商业应用： 高通量筛选技术在药物发现中具有商业应用价值 计划，并将销售给制药和生物技术公司。的 还可以开发用于体外诊断测定的技术 在生物医学、食品、乳制品和环境行业。

DESCRIPTION: (adapted from applicant's abstract) The aim of this SBIR proposal is to develop a novel, rapid, homogeneous assay for high throughput screening which is capable of detecting affinity interactions between antibody/antigen, receptor/ligand, and enzyme/substrate. High throughput screening (HTS) assays based on this new technology will have applications in pharmaceutical and biotechnology combinatorial drug discovery programs. The specific aims will be to develop novel reagents used in the assay, optimize assay conditions and develop instrumentation for performing the assay. The HTS assay combines the use of specially formulated photosynthetic membrane and fluorescence detection. The proposed work will focus on developing an HTS assay for identification of inhibitors of protein tyrosine kinases involved in signal transduction. An assay will be developed to identify inhibitors of src, an oncogenic protein tyrosine kinase overexpressed in certain cancer cells. PROPOSED COMMERCIAL APPLICATION: The high throughput screening assay has commercial applications in drug discovery programs and will be marketed to pharmaceutical and biotechnology companies. The technology can also be developed for application in vitro diagnostic assays for use in the biomedical, food, dairy and environmental industries.