INTERLEUKIN 1BETA EFECTS ON INGESTIVE BEHAVIOR

描述：这是一个学术研究增强的申请 要求为一个项目提供两年支持以检查其影响的奖励 卵巢激素对细胞因子抑制摄食量的影响 白介素1β。白介素1β(IL-1)是一种细胞因子， 急性期激活的单核细胞和巨噬细胞释放的 在感染或受伤后不久发生的反应。IL-1负责 对于许多局部和全身的炎症反应，也会诱发 一系列被称为“疾病行为”的症状(例如，身体不适， 疲倦，吃喝减少，对日常生活缺乏兴趣 活动)。当给大鼠注射IL-1时，会引起大鼠脑内 包括抑制食物和水摄取的行为。上一首 研究为免疫功能和性别差异提供了强有力的证据 性腺类固醇对它的调节。这些发现表明，在这两种情况下 无论是人类还是非人类动物，雌性动物的免疫力都比 男性。与这些观察结果相一致的是，行为 对IL-1的反应在大鼠的发情周期中波动。压抑的人 据报道，IL-1对动情期活性的影响比动情期更大。 非发情期的雌性。这些数据表明，IL-1的作用是 受卵巢激素的影响，并提示性腺之间的相互作用 类固醇和细胞因子可能会导致免疫反应的性别差异。 本研究的目的是评估雌二醇和 孕酮调节IL-1对肠道的抑制作用 行为，卵巢激素在其中发挥作用的一种行为系统 效果很强。从拟议的研究中获得的信息不会 只有助于我们理解细胞因子和性腺类固醇是如何 影响食物摄入量，但也将说明 内分泌、免疫和神经系统可能会导致性别差异。 在免疫反应中。目前正在提出两套实验方案。在 第一个实验是研究人员将评估雌二醇对 增强不同剂量对食物和水摄取的抑制作用 IP注射IL-1的数量。第二个实验将考察 孕酮在雌二醇与IL-1相互作用中的作用

DESCRIPTION: This is an application for an Academic Research Enhancement Award requesting two years of support for a project to examine the influence of ovarian hormones on the suppression of food intake by the cytokine interleukin 1 beta. Interleukin 1 beta (IL-1) is a cytokine that is released by monocytes and macrophages activated during the acute phase response which occurs soon after infection or injury. IL-1 is responsible for many of the local and general responses to inflammation and also induces a constellation of symptoms known as "sickness behavior" (e.g., malaise, fatigue, decreased eating and drinking, lack of interest in usual activities). When administered to rats, IL-1 causes profound alterations in behavior including a suppression of food and water intake. Previous research provides strong evidence for sex differences in immune function and its modulation by gonadal steroids. These findings indicate that, in both human and non-human animals, females are immunologically stronger than males. Consistent with these observations are the findings that behavioral responses to IL-1 fluctuate across the rat estrous cycle. The suppressive effect of IL-1 on activity has been reported to be greater in estrus than in non-estrus females. These data indicate that the effects of IL-1 are influenced by ovarian hormones and suggest that interactions between gonadal steroids and cytokines may contribute to sex differences in immune response. The goals of this study are to evaluate the ability of estradiol and progesterone to modulate the inhibitory effects of IL-1 on ingestive behavior, a behavioral system in which ovarian hormones are known to exert strong effects. Information obtained from the proposed research will not only contribute to our understanding of how cytokines and gonadal steroids influence food intake, but also will also illustrate how interactions among the endocrine, immune, and nervous systems may contribute to sex differences in the immune response. Two sets of experiments are being proposed. In the first experiment the investigator will evaluate the ability of estradiol to augment the inhibition on food and water intake produced by different doses of IP injections of IL-1. The second experiment will examine the role of progesterone in estradiol IL-1 interactions.