ENZYMOLOGY OF EGGSHELL FORMATION IN SCHISTOSOMA MANSONI

本实验室的长期目标是研究 曼氏血吸虫，特别是影响 雌性蠕虫 这些研究的一个核心需要是评估 雌蠕虫的发育状况。 蛋壳中的酶 蛋白质活化和交联仅在成人中表达， 性活跃的雌性蠕虫 酶测定和分子探针 将被开发来监测这些酶的表达。 这些 因此，酶将作为雌虫发育的标记。 本实验的另一个平行目标是应用 蠕虫生物化学与分子生物学基础研究 新的血吸虫病控制方法的产生。 血吸虫卵在病理学中具有核心重要性， 血吸虫病的传播。 更好地理解 生物化学和分子生物学事件参与生产鸡蛋， 女性的叛逆也可能导致小说的发展 化疗或抗肿瘤疫苗。 具体研究建议 目的是鉴定蛋壳中涉及的酶 雌性S. mansoni 我们的假设是蛋壳 形成的酶促途径类似于 节肢动物和软体动物的蛋壳形成和角质层硬化; 醌鞣和β-硬化的酶。 的作用 酪氨酸水解酶、酚氧化酶和过氧化物酶在线粒体中起作用， 蛋壳生产将被调查。 该项目的目标是 1)确定过氧化物酶在蛋壳形成中的作用; 2） 确定酪氨酸羟化酶在羟化 3）确定蛋壳蛋白质与蛋白质的关系。 将一些酚氧化酶转化为其它生物的酚氧化酶， 克隆其基因; 4）了解酚氧化酶在 卵壳蛋白的羟基化和氧化;和5） 测定酚氧化酶的分期和组织特异性表达 讨厌鬼

The long term objective of this lab is to study development in Schistosoma mansoni, especially factors affecting the development of female worms. A central need of these studies is a marker to assess the developmental status of female worms. The enzymes involved in eggshell protein activation and cross linking are only expressed in adult, sexually active female worms. Enzymatic assays and molecular probes will be developed to monitor the expression of these enzymes. These enzymes will therefore serve as markers for female worm development. A parallel objective of this lab is to apply the knowledge gained in fundamental investigations of worm biochemistry and molecular biology to the generation of novel methods of schistosomiasis control. Schistosome eggs are of central importance in the pathology and transmission of schistosomiasis. A better understanding of the biochemical and molecular events involved in the production of eggs in the female schistosome may also lead to the development of novel chemotherapies or a schistosome vaccine. The specific research proposed in this application is to identify the enzymes involved in eggshell formation in female S. mansoni. Our working hypothesis is that eggshell formation is schistosomes uses enzymatic pathways similar to those used for eggshell formation and cuticle hardening by arthropods and mollusks; the enzymes of quinone tanning and Beta-sclerotization. The role that tyrosine hydrolase, phenol oxidase, and peroxidase play in schistosome eggshell production will be investigated. The goals of this project are 1) to determine role of peroxidase in eggshell formation; 2) to determine the role of tyrosine hydroxylase in the hydroxylation of schistosome eggshell proteins; 3) to determine the relationship of schistosome phenol oxidase to the phenol oxidases of other organisms by cloning its gene; 4) to understand the role of phenol oxidase in the hydroxylation and oxidation of schistosome eggshell proteins; and 5) to determine the stage and tissue specific expression phenol oxidase in schistosomes.