EUKARYOTIC BIOLUMINESCENT INTEGRATED CIRCUIT SENSORS

描述(摘自申请者的摘要)：当前的葡萄糖生物传感器 技术依赖于固定化的酶-葡萄糖氧化酶生物反应器 由于葡萄糖氧化酶的存在而被证明是不可靠的 人体血液中的抑制物。一种利用世卫组织细胞的生物传感器 而不仅仅是细胞成分可能被证明对葡萄糖更可靠 监控。此外，白光检测对葡萄糖的响应 浓度。含LuxAB的真核生物发光细胞系 发光性致病杆菌的基因取代传统的Luc基因 在所有其他生物发光的真核生物中发现的系统。通过这样做，我们将 产生一种能够连续监测血糖的真核细胞系， 在线和实时的。LuxAB基因将被放入pLPK.Lucff，一个 能够感知葡萄糖的基于质粒的Luc基因系统。一次 成功构建并测试了葡萄糖敏感性，这些细胞 将被封装在包含光电探测器的集成电路上 这将监测发光活动。最终目标是 生产可靠的葡萄糖传感器，体积足够小，可用于日常工作 糖尿病患者的植入物。

DESCRIPTION (Adapted from applicant's abstract): Current glucose biosensor technology relies on immobilized enzyme-based glucose oxidase bioreactors which have proven unreliable due to the presence of a glucose oxidase inhibitor in the human bloodstream. A biosensor utilizing who cells rather than just cellular constituents may prove more reliable for glucose monitoring. Additionally, white-light detection in response to glucose concentrations. Eukaryotic bioluminescent cell line containing the luxAB gene from Xenorhabadus luminescens instead of the convention luc genetic systems found in all other bioluminescent eukaryotes. In so doing, we will produce a eukaryotic cell line capable of monitoring glucose continuously, on-line and in real-time. The luxAB gene will be placed in pLPK.LucFF, a plasmid-based luc genetic system capable of sensing glucose. Once successfully constructed and tested for glucose sensitivity, the cells will be encapsulated on an integrated circuit containing a photodetector that will monitor the luminescence activity. The ultimate goal is to produce a reliable glucose sensor small enough to be used for routine implantation in diabetic patients.