NEURAL BASIS OF TASTE ELICITED INGESTION AND REJECTION

描述：（改编自申请人的摘要）。 客观 的建议研究是确定神经基板在 啮齿动物的后脑，味觉刺激通过它引导摄食 行为尽管摄食行为的调节非常复杂， 复杂的，涉及广泛的区域的中枢神经系统， 味觉的基本调节功能， 不愉快的刺激在尾侧脑风暴中完成。 的 可口与不可口的区别是明显的， 去大脑动物对味觉刺激的摄食和排斥反应 准备工作神经解剖学研究表明多突触通路 从孤束吻侧核的味觉区 影响orthomatic核产生完善的组成部分， 摄入和排斥。这些通路与脑干部位重叠 与咀嚼、舔和吞咽的产生有关。 在啮齿类动物后脑中进行的一系列慢性记录实验将 确定在何种程度上不同区域的髓质网状 的形成涉及味觉引起的摄取和排斥。因为 舔、吞咽和排异的器官功能 共同的口腔肌肉组织，许多相同的中间神经元下 预期调查将涉及多种职能。的 给定神经元参与多个功能的情况将通过 将神经活动与肌电反应相关联， 参与不同功能的肌肉子集。时间序列分析， 尖峰计数、事件平均和尖峰触发平均都将被 用于评估网状结构神经元的反应特性 在特定的摄入和排斥过程中。一个基本 要解决的问题是与神经元相关的身份。 拒绝的口头阶段，“目瞪口呆”的反应。这些神经元 与产生舔周期，吞咽周期， 呼吸神经元，还是一组独特的神经元？一系列平行的 神经解剖学研究将使用双标记技术来识别 脑干的不同区域参与摄食， 拒绝，并进一步确定其与orthodox的连接 原子核。

DESCRIPTION: (adapted from the applicant's abstract). The objective of the proposed research is to determine the neural substrates in the rodent hindbrain through which gustatory stimuli guide ingestive behavior. Although the regulation of ingestive behavior is exceedingly complex, involving widespread regions of the central nervous system, the basic regulatory function of taste to determine palatable from unpalatable stimuli is complete in the caudal brainstorm. The discrimination of palatable from unpalatable is evident by normal ingestion and rejection responses to taste stimuli in decerebrate preparations. Neuroanatomical studies suggest that polysynaptic pathways from the gustatory region of the rostral nucleus of the solitary tract influence oromotor nuclei producing the consummatory components of ingestion and rejection. These pathways overlap with brainstem sites identified with the generation of mastication, licking and swallowing. A series of chronic recording experiments in the rodent hind brain will determine to what extent different regions of the medullary reticular formation are involved in taste elicited ingestion and rejection. Because the oromotor functions of licking, swallowing and rejection share a common oral musculature, many of the same interneurons under investigation are expected to be involved in multiple functions. The involvement of a given neuron in multiple functions will be assessed by correlating neural activity with electromyographic responses from a subset of muscles involved in diverse functions. Time series analyses, spike counts, event averaging and spike triggered averaging will all be used to assess the response properties of reticular formation neurons during specific components of ingestion and rejection. One fundamental question to be addressed is the identity of neurons associated with the oral phase of rejection, the "gape" response. Are these neurons associated with neurons generating a lick cycle, a swallow-cycle, respiratory neurons, or a unique set of neurons? A parallel series of neuroanatomical studies will use double labeling techniques to identify regions of the brainstem differentially involved in ingestion and rejection and to further determine their connectivity with the oromotor nuclei.