HIGH THROUGHPUT, LOW COST, SINGLE MOLECULE DNA SEQUENCIN

描述：SEQ正在开发单分子检测，鉴定， 操作和化学能力的目的，创造一个 高速、低成本DNA测序仪。 该测序方法包括： 分离和进行性核酸外切酶消化个体，长（50 kb）DNA链。 切割的核苷酸被固定在表面上， 然后被发现和识别。 重要的是，在 与其他测序方法不同，SEQ的方法使用天然DNA。 的 本申请寻求为该方案的那些方面提供资金， 在原理证明之前需要额外的研究和发明 示范;这些是：1）核苷酸光物理学和光化学，2） 核苷酸表面结合及其对核苷酸物理学和 化学，和3）核酸外切酶的持续加工能力及其裂解时间 分布 当与 排序方案，完成所代表的能力， 该应用程序的个别特定目标将使天然DNA单一 分子测序仪 当时，决议的大多数现在 关于这种定序器的效率和经济性的不确定性将 要负责

DESCRIPTION: SEQ is developing single-molecule detection, identification, manipulation and chemistry capabilities for the purpose of creating a high-speed, low cost DNA sequencer. This sequencing method consists of the isolation and processive exonucleolytic digestion of individual, long (50 kb) strands of DNA. The cleaved nucleotides are immobilized on a surface, in order, and subsequently detected and identified. Importantly, and in contrast to other sequencing methods, SEQ's approach uses native DNA. The present application seeks funding for those aspects of the scheme that require additional research and invention prior to a proof-of-principle demonstration; these are: 1) nucleotide photophysics and photochemistry, 2) nucleotide-surface binding and its effect on nucleotide photophysics and chemistry, and 3) exonuclease processivity and its cleavage-time distribution. When integrated with the advanced engineering portions of the sequencing scheme, the capabilities represented by the accomplishment of the individual specific aims of this application will enable a native-DNA single molecule sequencer. At that time, resolution of the majority of the present uncertainties regarding the efficiency and economy of such a sequencer will be answerable.