VEGETALLY LOCALIZED TRANSCRIPTS

描述(改编自申请人的摘要)：这是一份建议书 研究一个新的转录本家族的细胞定位。Dr。 埃特金的实验室已经克隆了一组基因，这些基因编码的是 非洲爪哇(Xlsirts)和非洲爪哇(Xlsirts)散布重复转录 热带非洲爪哇(Xtsirts)Sirt被组织为串联重复 由79-81个核苷酸重复组成的序列，重复3- 在不同的克隆中13次。没有开放的阅读框架，而且在 每个克隆的重复序列两侧都有不同的独特序列。vbl.使用 重复序列探针xlsirts的原位杂交分析 在第1期和第2期卵母细胞的线粒体云中检测到 它们被转移到植物的大脑皮层。Xlsirt 转录本是动植物两极分化的最早指标之一。 假说是它们可能作为结构组件发挥作用 植物皮层，并可能负责随后的定位 在植物皮质中的转录本，如xcat2或vg1。此外，它们的 这一区域的定位可能会影响生殖细胞等过程 影响腹背轴的大脑皮质旋转的形成 队形。其目的是：(1)测试细胞骨架元素的作用 在xlsirt转录本的定位和锚定中；(2)分析 顺式作用元件在xlsirts定位中的作用；(3) 分析xlsirts在其他RNAs本地化中的作用 作为xcat2、xcat3和vg1在卵母细胞中的表达及其功能的研究 在发育过程中；以及(4)从其他植物中克隆Xlsirt类似序列 如果蝇和小鼠等生物，并检查其定位 在一个异源系统中的Xlsirts。

DESCRIPTION (Adapted from the applicant's abstract): This is a proposal to study the cellular localization of a novel family of transcripts. Dr. Etkin's laboratory has cloned a family of genes that code for interspersed repeat transcripts from Xenopus laevis (xlsirts) and Xenopus tropicalis (xtsirts). Sirts are organized as tandem repeat sequences consisting of 79-81 nt repeats that are reiterated from 3 to 13 times in different clones. There are no open reading frames and in each clone the repeats are flanked by different unique sequences. Using in situ hybridization analysis with the repeat sequence probe the xlsirts were detected in the mitochondrial cloud of stage 1 and 2 oocytes after which they translocate to the vegetal cortical egion. The xlsirt transcripts are one of the earliest indices of animal-vegetal polarity. The hypothesis is that they may function as a structural component of the vegetal cortex and may be responsible for the subsequent localization of transcripts such as xcat2 or vg1 in the vegetal cortex.In addition, their localization in this region may influence processes such as germ cell formation or the cotical rotation which influences dorsal-ventral axis formation. The aims are: (1) to test the role of cytoskeletal elements in the localization and anchoring of xlsirt transcripts; (2) to analyze the role of the cis-acting elements in the localization of xlsirts; (3) to analyze the role of xlsirts in the localization of other RNAs such as xcat2, xcat3, and vg1 in oocytes and to determine their function during development; and (4) to clone xlsirt-like sequences from other organisms such as Drosophila and mouse and to examine the localization of xlsirts in a heterologous system.