ATM AND CELL DEATH IN THE NERVOUS SYSTEM

描述：这是一项新的建议，意在调查， 调控细胞凋亡，最终目的是阐明下游 ATM信令的效应器。这项建议是基于初步数据 表明ATM基因敲除中的特定分裂细胞群体 神经系统受到保护，不受电离促细胞凋亡的影响 辐射。应用程序的目的将是确定潜在的 生理上相关的激活剂(氧化应激、兴奋性毒性、 利用ATM-/-小鼠和神经元切断ATM信号通路 来源于这些动物的细胞培养；以鉴定 利用ATM参与ATM和神经系统的凋亡途径 -/-小鼠以及P53、Bcl-2和Bax-/-小鼠，以及来源于 最后，对这些动物进行详尽的调查和表征 ATM-/-小鼠神经系统的发育及ATM是否 对正常的程序性细胞死亡很重要。

DESCRIPTION: This is a new proposal, the intention is to investigate At, regulated apoptosis with the ultimate goal of elucidating downstream effectors of Atm signaling. The proposal is based on preliminary data indicating that specific populations of dividing cells in the Atm knockout nervous system are protected from the pro-apoptotic effects of ionizing radiation. The aims of the application will be to identify potential physiologically relevant activators (oxidative stress, excitotoxicity, axotomy) of the Atm signaling pathway by using Atm -/- mice and neuronal cell cultures derived from these animals; to identify components of the apoptotic pathway involving Atm and the nervous system by using the Atm -/- mice as well as p53, Bcl-2 and Bax -/- mice, and cultures derived from these animals and finally, to exhaustively investigate and characterize the developing nervous system in the Atm -/- mice and to ask whether Atm is important for normal programmed cell death.