PROTEIN/DNA INTERACTIONS IN THE YEAST GENOME

描述：(申请人摘要)酵母基因组序列现已知， 一项重大技术成就，为 研究最简单的真核生物的细胞生物学。了解以下内容 体内调节蛋白和结构蛋白与DNA的相互作用 重要的应用-酵母DNA序列对其功能的影响。这 提案涉及如何实现这一目标。它建立在新兴的基础上 DNA甲基转移酶在水稻蛋白质-DNA相互作用研究中的应用 通过开发方法来实现对活细胞的解析(&lt；l0 base 对酿酒酵母延伸区相互作用的研究 基因组。它描述了能够进行定量评估的方法 利用适当的自动化技术实现蛋白质-DNA相互作用 用于酵母基因组的大区域，并可输出到更大的真核生物。 为了说明这种方法的实用性，我们将绘制染色质 约一半的染色体的组织。比较部分染色体 结构与经典方法确定的结构一致。许多其他人 实验室对染色质的结构和作用都非常感兴趣 在DNA功能和调节蛋白与DNA的相互作用中。至 为了方便这些科学家的研究，我们提出了一项服务，将 提供酵母基因组其他区域的染色质图谱数据 利息。

DESCRIPTION: (Applicant's abstract) The yeast genome sequence is now known, a major technical accomplishment that offer unprecedented opportunities for study of the cell biology of the simplest eukaryote. Knowledge of the interactions of regulatory and structural proteins with DNA in vivo is important for application-of the yeast DNA sequence to its function. This proposal addresses how to accomplish that goal. It builds on emerging applications of DNA methyltransferases to map protein-DNA interactions in living cells by developing methodology that will allow resolution (<l0 base pairs) study of interactions over extended regions of the S. cerevisiae genome. It describes methodology which will allow quantitative assessment of protein-DNA interactions with automated techniques that are appropriate for large regions of the yeast genome and exportable to larger eukaryotes. To illustrate the utility of the approach, we will map the chromatin organization of about half of chromosome III. Comparing portions of the structure with that determined by classical methods. Many other laboratories are highly interested both in chromatin structure and its role in DNA function and in interactions of regulatory proteins with DNA. To facilitate the research of these scientists, we propose a service which will provide chromatin mapping data for other regions of the yeast genome of interest.