STRUCTURE OF VERTEBRATE STRIATED MUSCLE W/ ADDED EXOGENOUS MYOSIN SUBFRAGMENT 1

海德堡肌肉组织的长期目标是了解 通过系统地建立肌肉的结构和功能 结构与其组件的结构不同。通过拟合由以下各项组成的模型 在肌肉中发现的单个蛋白质的晶体结构 从去皮的肌肉纤维束获得的纤维衍射图， 人们可以看到当这些结构处于 大分子原位组装。我们利用BioCAT的设施 获得非常高质量的过度伸展兔纤维图案 外源肌球蛋白头部标记的肌肉(S-1)及其催化结构域 在ADP存在和不存在的情况下从Dictostelius模拟 产生“强力冲刺”的力量。我们能够检测到强度 纤维图案的变化可能与 这些物种的区域结构。(Poole等人，1999)。我们是 目前正在完善我们的模型，为出版做准备。

The long term goal of the Heidelberg muscle group is to understand the structure and function of muscle by systematically building up the structure from that of its components. By fitting models composed of the crystal structures of the individual proteins found in muscle to fiber diffraction patterns obtained from skinned muscle fiber bundles, one can see how these structures change when they are in a macromolecular assembly in situ. We used the BioCAT facility to obtain very high quality fiber patterns of over-stretched rabbit muscle labeled with exogenous myosin heads (S-1) and catalytic domains from Dictostelium in the presence and absence of ADP to mimic the force producing "power stroke". We were able to detect intensity changes in the fiber pattern that can be related to changes in the domain structure of these species. (Poole et al., 1999). We are presently refining our models in preparation for publication.