CORE--PREPARATION, STORAGE, AND SHIPPING OF PLANT EXTRACTS AND ASSAYS

这个核心设施将负责进行准备工作， 植物提取物的储存和运输，以及大部分分析工作的运行 在NPDDG内。这将包括 。植物材料的浸泡.称重、包装、运输和 植物提取物的盘点 。检测提取物中是否含有DNA损伤剂 拓扑异构酶抑制剂和DNA聚合酶β抑制剂，以支持 节目2. 。纯化DNA拓扑异构酶I和DNA聚合酶β的分离 化验工作 。从NCI天然产物仓库收到的样品的筛选 。通过开发/应用来指导提取物的选择 支持实验室项目2的二次鉴别分析 。在实验室计划3中指导提取物的分级

This Core facility will be responsible for carrying out the preparation, storage and shipping of plant extracts, and much of the assay work run within the NPDDG. This is to include . soaking of plant materials; weighing, packaging, shipping and inventorying of plant extracts . testing of extracts for the presence of DNA damaging agents, DNA topoisomerase inhibitors and DNA polymerase beta inhibitors, in support of Programs 2. . isolation of purified DNA topoisomerase I and DNA polymerase beta for assay work . screening of samples received from NCI natural products repository . guiding the choice of extracts by the development/application of secondary discrimination assays in support of Laboratory Programs 2 . guiding the fractionation of extracts within Laboratory Program 3