CORE--LABORATORY FACILITY

STD-CRC实验室核心将：l)为STD-CRC内开展临床、流行病学和行为项目的调查人员提供必要的专业微生物学和实验室专业知识；2)促进基础科学或分子生物学的新进展迅速应用于研究中心的相关临床和流行病学项目；3)为其他研究者研究性病提供国内和国际参考实验室；4)保持衣原体和淋病登记，继续评估与再感染相关的因素。核心中心提供的实验室专业知识将包括高度专业化的实验室测试，即使在其他研究实验室也不经常提供这些测试，但这些测试对于拟议的流行病学或临床研究的成功是不可或缺的。此类检测的例子包括检测沙眼衣原体血清型；淋球菌原型和血清型的测定；衣原体PCR和LCR检测；淋病奈瑟菌脉冲场凝胶电泳分析选定衣原体菌株MOMP核苷酸序列；沙眼衣原体微IF血清学检测及尿标本特异性HPV PCR检测。在一个中央核心实验室提供这些和其他检测，可确保由技术上有经验的调查人员进行检测，通过规模经济降低成本，并使进行行为和流行病学研究的研究人员能够获得这些检测，否则他们可能无法获得实验室合作者的帮助。这种可用性将促进在行为研究中使用微生物终点。虽然支持基础科学工作不是实验室核心的目的，但核心将积极寻求将适当的技术从基础科学实验室转移到相关的临床和流行病学项目。例如，我们建议进一步开发和实施一种类型特异性HPV PCR，该PCR将允许从尿液样本进行诊断，从而促进基于人群的研究。在实验室核心内进行的程序将作为国家和国际资源提供给其他研究人员，使他们无需开发昂贵且技术要求高的测试。

The STD-CRC laboratory core will: l) provide necessary specialized microbiological and laboratory expertise for investigators performing clinical, epidemiological and behavioral projects within the STD-CRC; 2) facilitate the rapid application of new advances in basic sciences or molecular biology to relevant clinical and epidemiological projects within the SDC-CRC; 3) serve as a national and international reference laboratory for other investigators studying STDs; and 4) maintain a chlamydia and gonorrhea registry to continue assessment of factors associated with reinfection. The laboratory expertise provided in the core will consist of highly specialized laboratory tests that are not routinely available even in other research laboratories but are integral to the success of proposed epidemiological or clinical research. Examples of such tests include determination of C. trachomatis serovars; determination of gonococcal auxotypes and serotypes; PCR and LCR tests for chlamydia; pulse field gel electrophoresis (PFGE) analysis of N. gonorrhoeae; MOMP nucleotide sequences of selected chlamydia strains; Micro IF serology for C. trachomatis and type specific HPV PCR on urine specimens. Provision of these and other tests in a central core laboratory ensures that they will be performed by technically experienced investigators, reduces costs by economy of scale, and makes these tests available to researchers conducting behavioral and epidemiological studies who might otherwise not have access to laboratory collaborators. Such availability will foster the use of microbiological endpoints in behavioral studies. While it is not the purpose of the laboratory core to support basic science work, the core will actively seek to transfer appropriate technology from basic science laboratories to relevant clinical and epidemiological projects. For example, we propose the further development and implementation of a type specific HPV PCR that will allow diagnosis from urine specimens, thus facilitating population based studies. The procedures performed within the laboratory core will be available as a national and international resource for other investigators, eliminating the need for them to develop expensive and technically demanding tests.