DEVELOPMENT OF 1H METABOLITE IMAGING FOR CANCER

描述(摘自摘要)：人肿瘤的活体核磁共振波谱 芳香区实际上是一个未开发的区域。含有以下成分的化合物 芳香环在生物化学中占有众多而重要的地位 生命系统和癌症的过程。例如，派生的 嘌呤和嘧啶是核苷酸、DNA和RNA的重要组成部分， 肌肉中的芳香族质子也包括对pH敏感的 肌肽的咪唑质子。初步高空间分辨率 结果显示，大脑中假定的核苷酸信号 在肿瘤中显著减少。此外，强磁化 大脑芳香族质子的转移(MT)效应已经被 观察到的。因为MT的产生依赖于 两个不同质子池之间的磁化交换，这取决于 关于组织的生物物理性质可能不同于 正常组织、肿瘤和坏死。因此，在活体内，人类的~1H MRS 芳香区可能与活体1H MRS(即 上视野区域中的信号)通过提供新的和 用于肿瘤诊断/预后的独立参数。高 空间分辨率对于区分不同的病理和 用于诊断的正常组织。然而，较长的获取时间， 短T2、低浓度和脂肪/水抑制是主要原因 高空间分辨率活体~1H芳香族成像面临的挑战 癌症。新型“回声滤光器”的进一步开发/优化 技术可以同时解决这些技术挑战。这个 这项工作的长期目标是评估芳香族质子的作用 在肿瘤的诊断/预后方面。具体目标是：1)进一步 发展回声滤光器直接成像技术(即代谢物 没有化学位移演化期的成像技术) 芳香质子；2)建立正常组织的基线 肿瘤~1H-MRS体内研究芳香区的获得 芳香族质子在脑肿瘤和软组织中的初步临床资料 探讨该方法在组织肉瘤诊断中的应用 预测工具。这项研究的积极结果将为 更有针对性的临床试验在肿瘤的诊断/预后中使用 芳香族质子和MT效应作为新的参数。

DESCRIPTION (taken from abstract): In vivo 1H MRS of human cancers in the aromatic region is virtually an unexplored area. Compounds with aromatic rings occupy numerous and important positions in biochemical processes of living systems and cancers. For example, derivatives of purine and pyrimidine are essential parts of nucleotides, DNA and RNA, and the aromatic protons in muscle also include the pH sensitive imidazole proton of carnosine. Preliminary high spatial resolution results have revealed that the putative nucleotide signal in the brain is dramatically reduced in tumors. In addition, strong magnetization transfer (MT) effects in the aromatic protons of the brain have been observed. Since the generation of MT relies on the presence of magnetization exchange between two different proton pools, it depends on the biophysical properties of the tissue which may differ among normal tissues, tumors and necrosis. Thus, in vivo 1H MRS of humans in the aromatic region may compliment `traditional` in vivo 1H MRS (i.e. signals in the up-field region) of lesions by providing new and independent parameters for the diagnosis/prognosis of tumors. High spatial resolution is essential to differentiate various pathologic and normal tissues for decisive diagnosis. However, long acquisition time, short T2, low concentration, and lipid/water suppression are major challenges to high spatial resolution in vivo 1H aromatic imaging of cancers. Further development/optimization of the novel `echo-filter` technique can simultaneously address these technical challenges. The long term goal of the work is to evaluate the role of aromatic protons in the diagnosis/prognosis of tumors. The specific aims are: 1) further develop the echo-filter direct imaging technique (i.e. the metabolite imaging technique without a chemical shift evolution period) for aromatic protons; 2) establish the baseline of normal tissues in the aromatic region for in vivo 1H MRS study of cancers 3) obtain preliminary clinical data of aromatic protons in brain tumors and soft tissue sarcomas to explore the utility of this method as a diagnosis/ prognostic tool. Positive results of this study will pave the way for more focused clinical trials in the diagnosis/prognosis of tumors using aromatic protons and MT effects as new parameters.