Experimental Analysis of Intracerebral Immunosurveillance Mechanism and Its Clinical Application to Management of Brain Neoplasms

1.基础研究：(1)主要组织相容性复合体(MHC，14-2)阳性的肿瘤细胞移植到脑内可被细胞毒性T淋巴细胞(CTL)以一种MHC依赖的方式排斥，而MHC阴性的肿瘤细胞由于缺乏自然杀伤细胞(NK细胞)介导的脑内自然抵抗，可逃脱CTL介导的免疫监视而在脑内生长。(2)我们证明了干扰素-β对小鼠恶性星形细胞瘤细胞内钙动员没有明显影响，而细胞因子对肿瘤细胞的增殖有抑制作用。临床研究：(1)基于微创神经外科的理念，设计了超声和神经内窥镜辅助的脑肿瘤显微外科手术方式，以提高脑肿瘤患者的术后生活质量。(2)观察儿童和老年脑肿瘤患者经多学科治疗后存活5年以上的远期疗效和生活质量。儿童和老年患者放疗后的脑/脊髓损伤应引起重视，特别要注意并发症的发生。

1. Basic Research :(1) It was suggested that major histocompatibility complex (MHC, 14-2 in mice)-positive tumor cells grafted into the brain may be rejected by cytotoxic T lymphocytes (CTL) in an MHC-dependent manner, whereas MHC-negative tumor cells can escape from CTL-mediated immunosurveillance and grow progressively in the brain, due to absence of intracerebral natural resistance mediated by natural killer (NK) cells.(2) We demonstrated that interferon- beta had no apparent effect on intracellular calcium mobilization of the murine malignant astrocytoma cells, while there was an inhibitory action of the cytokine on the tumor cell proliferation.2. Clinical Research :(1)We devised an operative modality with ultrasound and neuroendoscope-assisted microsurgical techniques based upon the concept of minimally invasive neurosurgery for patients with brain neoplasms to enhance the postoperative quality of life.(2) We examined the long-term outcome and quality of life in the pediatric and geriatric patients with brain neoplasms, surviving more than 5 years after multidisciplinary treatments. With special reference to complication, more attention should be exercised to the brain/spinal cord injuries following radiotherapy of pediatricand geriatric patients.