Experimental Analysis of Intracerebral Immunosurveillance Mechanism and Its Clinical Application to Management of Brain Neoplasms

脑内免疫监视机制的实验分析及其在脑肿瘤治疗中的临床应用

• 批准号: 09470295
• 负责人: YAMASAKI Toshiki
• 金额: $ 8.77万
• 依托单位: SHIMANE MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470295/
• 关键词: brain neoplasm; gene regulation; major histocompatibility complex antigen; immune response; neuroendoscope; ultrasound; microsurgery; minimally invasive surgery

1. Basic Research :(1) It was suggested that major histocompatibility complex (MHC, 14-2 in mice)-positive tumor cells grafted into the brain may be rejected by cytotoxic T lymphocytes (CTL) in an MHC-dependent manner, whereas MHC-negative tumor cells can escape from CTL-mediated immunosurveillance and grow progressively in the brain, due to absence of intracerebral natural resistance mediated by natural killer (NK) cells.(2) We demonstrated that interferon- beta had no apparent effect on intracellular calcium mobilization of the murine malignant astrocytoma cells, while there was an inhibitory action of the cytokine on the tumor cell proliferation.2. Clinical Research :(1)We devised an operative modality with ultrasound and neuroendoscope-assisted microsurgical techniques based upon the concept of minimally invasive neurosurgery for patients with brain neoplasms to enhance the postoperative quality of life.(2) We examined the long-term outcome and quality of life in the pediatric and geriatric patients with brain neoplasms, surviving more than 5 years after multidisciplinary treatments. With special reference to complication, more attention should be exercised to the brain/spinal cord injuries following radiotherapy of pediatricand geriatric patients.

1.基础研究：（1）主要组织相容性复合体（MHC，14-2）阳性的肿瘤细胞移植到脑内后，可被细胞毒性T淋巴细胞（CTL）以MHC依赖的方式排斥，而MHC阴性的肿瘤细胞由于缺乏自然杀伤（NK）细胞介导的脑内自然抵抗，可逃避CTL介导的免疫监视，在脑内进行性生长。(2)结果表明：干扰素-β对小鼠恶性星形细胞瘤细胞内钙离子动员无明显影响，但对肿瘤细胞增殖有抑制作用.临床研究：（1）根据微创神经外科的理念，设计了一种超声和神经内镜辅助显微外科技术治疗脑肿瘤的手术方式，以提高患者术后生活质量。(2)我们研究了多学科治疗后存活超过5年的儿童和老年脑肿瘤患者的长期结局和生活质量。儿童和老年患者放疗后的脑/脊髓损伤应引起高度重视，并应特别注意并发症。

项目成果

Yamasaki T: "11th International Congress of Neurological Surgery" Monduzzi Editore, 5 (1997)

Yamasaki T：“第 11 届国际神经外科大会”Monduzzi Editore，5 (1997)

Kamei T: "Influence of forehead exposure to red photodiode light on NK activity." Photomed.and Photobiol. 19. 145-148 (1997)

Kamei T：“前额暴露于红色光电二极管光对 NK 活性的影响。”

山崎俊樹: "小児脳神経の外科 -Standard and Modified Techniques- 第1版" 医学書院, 13 (1998)

Toshiki Yamazaki：“小儿神经外科 - 标准和改良技术 - 第 1 版” Igaku Shoin，13 (1998)

山崎俊樹: "神経皮膚症候群の手術" 医学書院(印刷中),

山崎俊树：“神经皮肤综合征手术”Igaku Shoin（出版中），

Kamei T: "Forehead exposure of pulsed light at the frequency range of alpha wave increases NK activity" Medical & Biological Engineering & Computing. 35. 592-592 (1997)

Kamei T：“额头暴露在 α 波频率范围的脉冲光会增加 NK 活性” 医学