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本研究的目的是改进前白三烯的分子分析方法。 通过内窥镜活检获得的肿瘤和肿瘤组织。长的- 这项研究的长期目标是开发一种执行分子 只能从有限大小的样本中提取的病变的分析 已采样。内窥镜活检通常从胃肠道获得。 但有两个主要问题阻碍了它们的应用 分子分析。现有技术的主要问题是 需要仅依靠肉眼检查来确定哪些组织可以 用于分子分析。第二个问题是无法执行 对获得的小尺寸组织样本进行基因测试 来自内窥镜活组织检查。这项提案将评估两个人的能力 改进肿瘤组织中突变检测的技术 使用内窥镜活检材料。第一种技术是“光学技术” 利用激光诱导荧光(LIF)和漫反射技术进行活组织检查 光谱学，允许选择适当的肿瘤组织 以供分析。这种装置可以立即定位肿瘤。 组织具有高水平的精度，这允许对 最适合分子分析的组织。样品将会是 使用一种名为变性高压液体的新技术进行加工 快速、灵敏和自动化的色谱学(DHPLC) 用于筛查遗传异常的技术。我们会提议测试 这些技术在巴雷特食道，一种癌前上皮组织中 在远端的食道。这些患者都有共同的特点 可以用这些技术检查的分子异常。我们 会将组织的分子分析结果与 光学活检与标准技术获得的结果进行比较。在……里面 此外，我们还将评估DHPLC和直接基因检测的能力 测序以快速筛选和识别突变。这是意料之中的 这些技术的结合可以大大增加 从有限的组织样本中检测分子异常的能力 通过内窥镜检查获得。这些技术有发展的潜力。 对任何有限大小的组织样本进行的分子分析。

The aim of this study is to improve the molecular analysis of pre- neoplastic and neoplastic tissue obtained by endoscopic biopsy. The long- term goal of this study is to develop a method of performing molecular analysis of lesions from which only limited sized specimens can be sampled. Endoscopic biopsies are routinely obtained from gastrointestinal neoplasms but two major problems have prevented their utilization for molecular analysis. The primary problem with existing technology has been the need to rely solely on visual inspection to determine which tissue can be used for molecular analysis. The second is the inability to perform genetic testing on the small size of tissue specimens that are obtained from endoscopic biopsies. This proposal will assess the ability of two techniques to improve the detection of mutations in neoplastic tissues using endoscopic biopsy material. The first technique is an "optical biopsy" using laser induced fluorescence (LIF) and diffuse reflectance spectroscopy that allows the selection the appropriate neoplastic tissue for analysis. This device allows immediate localization of the neoplastic tissue with a high level of accuracy, which permits the sampling of the most appropriate tissue for molecular analysis. The samples will be processed using a new technique termed Denaturing High Pressured Liquid Chromatography (DHPLC) which is a rapid, sensitive, and automated technique for screening genetic abnormalities. We would propose to test these techniques in Barrett's esophagus, a pre-malignant epithelial tissue of the distal esophagus. These patients have common well-characterized molecular abnormalities that can be examined with these techniques. We will compare the rests of molecular analysis of tissue obtained with optical biopsy compared with obtained with standard techniques. In addition, we will assess the ability of DHPLC followed by direct gene sequencing to rapidly screen and identify mutations. It is anticipated that the combination of these techniques can substantially increase the ability to detect molecular abnormalities from limited tissue samples obtained through endoscopy. These techniques have the potential to advance the molecular analysis of any tissue samples of limited size.