ANALYSIS OF PAROTID GLAND 4B-GALACTOSYLTRANSFERASE

长期注射β-肾上腺素能激动剂， 提高细胞内cAMP水平和某些饮食变化， 细胞肥大和增生的大鼠腮腺。 此外，高尔基体的比活性增加， 标志酶4 β-半乳糖基转移酶。 这种活动的变化是 伴随着这种酶的亚细胞定位的改变， 质膜 上调和表面的重要性 在实验中， 与这种酶相互作用显示出严重抑制腮腺 防止体外或体内发生细胞肥大和增生 vivo. 大量的报道表明，细胞表面Gal T 'ase活性可能与细胞的粘附、分化和增殖有关。 肿瘤发生 转移酶调节的分子研究， 对于了解腮腺和其他组织的生长控制至关重要 组织中 我们对唾液腺发育的基本认识， 分化是由生理和组织学指导的 意见。 我的实验室提供了新的见解 腺泡细胞生长的生物化学和分子基础， 4 β-半乳糖苷酶的cDNA克隆。 我们的长期目标是了解 Gal T 'ase基因的复杂调控及其如何介导细胞凋亡 增长 RCDA将允许我有时间进行几项活动， 会促进我的研究发展 在颁奖过程中， 我们建议与其他唾液腺合作， 细胞生物学方面的专家。 我们的兴趣之一是 半乳糖苷酶在与细胞外基质的发育相互作用中的作用 基质及其对细胞迁移的贡献。 RCDA还将 请允许我有时间扩大我与教师的互动， 牙科学院。 对人类病理学相关性的追求 涉及组织增生的口腔将需要关闭 与临床和研究学院的合作。 这些经验 将是非常有价值的，可以应用在我未来的研究， 腮腺的生长和发育。

Chronic injections of beta-adrenergic agonist, agents that specifically raise intracellular cAMP levels and certain dietary changes, produce cell hypertrophy and hyperplasia in the rat parotid gland. Additionally, there is an increase in specific activity of the Golgi marker enzyme 4beta-galactosyltransferase. This change in activity is accompanied by an altered subcellular localization of this enzyme to the plasma membrane. The importance of up-regulation and surface localization became apparent in experiments in which agents that interact with this enzyme were shown to severely inhibit parotid gland cell hypertrophy and hyperplasia from taking place either in vitro or in vivo. Numerous reports have shown the alteration of cell surface Gal T'ase activity might be involved in cell adhesion, differentiation and tumorogenesis. Molecular studies on the regulation of the transferase, are crucial to understanding growth control in parotid and other tissues. Our basic understanding of salivary gland development and differentiation are guided by physiological and histological observations. My laboratory has provided new insights into the biochemical and molecular basis of acinar cell growth by having isolated a cDNA clone to 4beta-Gal T'ase. Our long-term goal is to understand the complex regulation of the Gal T'ase gene and how it mediates cell growth. The RCDA would allow me time to pursue several activities which would enhance my research development. During the course of the award, we propose to initiate collaborations with other salivary gland researchers with expertise in cell biology. One of our interests is the role Gal T'ase plays in developmental interactions with extracellular matrix and its contribution to cell migration. The RCDA would also allow me time for expanding my interactions with the faculty in the College of Dentistry. The pursuit of correlations in human pathologies of the oral cavity involving tissue hyperplasia will require close cooperation with the clinical and research faculty. These experiences would be extremely valuable and could be applied in my future studies of parotid gland growth and development.