PATHOGENIC ROLES OF THE ALVEOLAR MACROPHAGE IN ASTHMA

哮喘是一种具有重要临床和经济意义的疾病， 影响了大约900万年轻人，否则健康的人。 炎症是哮喘的一个重要方面，但许多 尚未研究炎症的潜在决定因素。 肺泡巨噬细胞（AM）的作用将通过以下方式确定： 这些建议的研究。 这些细胞可能有助于 气道炎症和高反应性的产生高 能量氧物种（HEOS），释放趋化因子， 炎性细胞如嗜酸性粒细胞和嗜中性粒细胞，以及 阐述促进组胺释放的因素。 过敏 哮喘受试者将在治疗前后进行研究。 支气管激发（BP）与它们敏感的抗原， AM经支气管肺泡灌洗恢复。 HEOS代谢 将通过超氧化物的生化测定来确定 和过氧化氢，以及鲁米诺增强的 化学发光反应 释放趋化素， 将通过标准微趋化性测定粒细胞 测定和AM衍生的组胺释放活性， 组胺的荧光分析。 过敏性哮喘的结果 BP后的患者将与正常血压的患者进行比较。 对照组和过敏性鼻炎患者，并以其自身值 在BP之前

Asthma is a disease of great clinical and economic importance, afflicting some nine million young, otherwise healthy individuals. Inflammation is an important aspect of asthma, but many potential determinants of inflammation have not been studied. Roles of the alveolar macrophage (AM) will be determined by these proposed studies. It is likely that these cells contribute to airway inflammation and hyperreactivity by generation of high energy oxygen species (HEOS), release of chemotactins for inflammatory cells such as eosinophils and neutrophils, and elaboration of factors promoting histamine release. Allergic asthmatic subjects will be studied before and after bronchoprovaction (BP) with antigen to which they are sensitive, and AM recovered by bronchoalveolar lavage. HEOS metabolism will be established by biochemical determinations of superoxide and hydrogen peroxide in addition to luminol-enhanced chemiluminescence response. Release of chemotactins for granulocytes will be determined by standard microchemotaxis assays, and AM-derived histamine releasing activity by fluorimetric analysis of histamine. Results from allergic asthma patients after BP will be compared to those obtained from normal controls and allergic rhinitis patients, and to their own values prior to BP.