HPV: BIOLOGY, CLINICAL SIGNIFICANCE AND EPIDEMIOLOGY

这项研究与人类乳头瘤病毒的作用有关。 在癌症的病因学方面。个别项目将解决 这些病毒感染的流行病学及其机制 通过它宿主细胞的肿瘤性转化 调解过的。病例对照研究将评估这一假设 感染HPV与癌症风险增加有关， 其他因素可能对引发剂/促进剂起作用 机制。生化和血清学支持 流行病学研究将在 研究计划，其方法包括分子 原位杂交和硝酸纤维蛋白印迹， 免疫过氧化物酶染色、抗体检测和分子生物学 克隆表达载体以提供确定的病毒抗原 和抗体。

This study is concerned with the role of human papillomaviruses in the etiology of cancer. The individual projects will address the epidemiology of these virus infections and the mechanisms through which neoplastic transformation of host cells is mediated. Case-control studies will evaluate the hypothesis that infection with HPV is related to an increased risk of cancer and that other factors may contribute to an initiator/promoter mechanism. Biochemical and serological support for the epidemiological studies will be utilized and developed in the research program, the methods for which include molecular hybridization in situ and with nitrocellulose blotting, immunoperoxidase staining, antibody evaluation and molecular cloning in expression vectors to provide defined viral antigens and antibodies.