A TRIAL OF PHYLLANTHUS AMARUS IN PEKIN DUCKLINGS

40%的人慢性感染乙肝病毒(乙肝病毒) 原发性肝细胞癌(PHC)的终生风险和15% 死于肝硬变的风险。目前，还没有安全有效的方法。 治疗急性或慢性乙肝病毒感染。土拨鼠 慢性感染土拨鼠肝炎病毒(WHV)是一种很好的 人类慢性乙肝感染的“模型”。他们有很高的风险 发展PHC。在这个项目中，土拨鼠长期感染了 WHV将被用来检验WHV的土拨鼠携带者 用热带植物苦叶下珠的提取物处理后 患上原发性肝细胞癌的风险显著降低 (PHC)比服用安慰剂的对照组更好。 苦瓜提取物对小鼠体内HBVDNA聚合酶、WHV和DHBVDNA聚合酶的抑制作用 体外培养。在体内，在最近感染的携带者中，病毒似乎是 治疗后被消除，治疗停止后不再返回。 在长期携带者中，环状病毒的数量明显减少。 在治疗之后。 为了促进对这一现象的生物学理解，在 除了对PHC发展的观察外，还对 WHV感染的血清学参数、早期的血清标志物 致癌(甲胎蛋白、铁蛋白)和复制形式 肝组织中的WHV DNA将被制造出来。 根据我们早期研究得出的估计，我们计算出 20只接受治疗的动物和20只对照动物将给予显著的力量来测试 假设。它们将被观察2年； 项目为期3年。 在以前的论文和赠款申请中， 提取物经鉴定为P.niruru。它现在已经被重新- 被归类为苦豆腐霉属。

Chronic infection with hepatitis B virus (HBV) imparts a 40 percent lifetime risk of primary hepatocellular carcinoma (PHC) and a 15 percent risk of death from cirrhosis. At present, there is no safe effective treatment for either acute or chronic infection with HBV. Woodchucks chronically infected with woodchuck hepatitis virus (WHV) are a good "model" for chronic HBV infection in humans. They have a very high risk of developing PHC. In this project, woodchucks chronically infected with WHV will be used to test the hypothesis that woodchuck carriers of WHV treated with an extract of the tropical plant Phyllanthus amarus will have a significantly lower risk for primary hepatocellular carcinoma (PHC) than controls treated with placebo. Extracts of P. amarus inhibit the DNA polymerase of HBV, WHV and DHBV in vitro. In vivo, in recently infected carriers, the virus appears to be eliminated after treatment and does not return when treatment is stopped. In long-term carriers, the amount of cirus is markedly decreased following treatment. To contribute to a biological understanding of the phenomenon, in addition to the observations on development of PHC, measurements of the serological parameters of WHV infection, serological markers of early carcinogenesis (alpha fetoprotein, ferritin), and replicating forms of WHV DNA in liver tissues will be made. Based on estimates derived from our earlier studies, we calculated that 20 treated and 20 control animals will give significant power to test the hypothesis. They will be observed for 2 years; the total length of the project is 3 years. In previous papers and the grant application the plant from which the extract is derived was identified as P. niruru. It has now been re- classified as P. amarus.